Immunology Antibodies 2

Anti-ATF7 Antibody (CAB7580)

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SKU:
CAB7580
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-ATF7 Antibody
Antibody SKU:CAB7580
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human ATF7 (NP_001193611.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:BT-474, SW620, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human ATF7 (NP_001193611.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGDD RPFV CNAP GCGQ RFTN EDHL AVHK HKHE MTLK FGPA RTDS VIIA DQTP TPTR FLKN CEEV GLFN ELAS SFEH EFKK AADE DEKK ARSR TVAK KLVV FRPR LFLL CFGI IFLI G
Gene ID:11016
Uniprot:P17544
Cellular Location:Cytoplasm, Nucleus, nucleoplasm
Calculated MW:13kDa/33kDa/49kDa/50kDa/51kDa/52kDa
Observed MW:53kDa
Synonyms:ATF7, ATFA
Background:
UniProt Protein Function:Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.
UniProt Code:P17544
NCBI GenInfo Identifier:12643393
NCBI Gene ID:11016
NCBI Accession:P17544.2
UniProt Secondary Accession:P17544,Q13814, Q8IVR8, Q9UD83, A5D6Y4, B2RMP1, B4DQL4
UniProt Related Accession:P17544
Molecular Weight:51,757 Da
NCBI Full Name:Cyclic AMP-dependent transcription factor ATF-7
NCBI Synonym Full Names:activating transcription factor 7
NCBI Official Symbol:ATF7  
NCBI Official Synonym Symbols:ATFA  
NCBI Protein Information:cyclic AMP-dependent transcription factor ATF-7
UniProt Protein Name:Cyclic AMP-dependent transcription factor ATF-7
UniProt Synonym Protein Names:Activating transcription factor 7; Transcription factor ATF-A
Protein Family:Cyclic AMP-dependent transcription factor
UniProt Gene Name:ATF7  
UniProt Entry Name:ATF7_HUMAN
Anti-ATF7 Antibody (CAB7580)Western blot analysis of extracts of various cell lines, using ATF7 antibody (CAB7580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-ATF7 Antibody (CAB7580)Immunofluorescence analysis of A549 cells using ATF7 antibody (CAB7580). Blue: DAPI for nuclear staining.
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