Epigenetics & Nuclear Signaling Antibodies 1
Anti-ATF6 Antibody (CAB0202)
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- SKU:
- CAB0202
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-ATF6 Antibody |
| Antibody SKU: | CAB0202 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ATF6 (NP_031374.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, Mouse brain, Mouse kidney, HeLa, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ATF6 (NP_031374.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGEP AGVA GTME SPFS PGLF HRLD EDWD SALF AELG YFTD TDEL QLEA ANET YENN FDNL DFDL DLMP WESD IWDI NNQI CTVK DIKA EPQP LSPA SSSY SVSS PRSV DSYS STQH VPEE LDLS SSSQ MSPL SLYG ENSN SLSS AEPL KEDK PVTG PRNK TENG LTPK KKIQ VNSK PSIQ PKPL LLPA AP |
| Gene ID: | 22926 |
| Uniprot: | P18850 |
| Cellular Location: | Endoplasmic reticulum membrane, Nucleus, Single-pass type II membrane protein |
| Calculated MW: | 74kDa |
| Observed MW: | 100KDa |
| Synonyms: | ATF6, ACHM7, ATF6A |
| Background: | This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. |
| UniProt Protein Function: | ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily. |
| UniProt Protein Details: | Protein type:Transcription factor; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q23.3 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; integral to endoplasmic reticulum membrane; membrane; nuclear envelope; nucleoplasm; nucleus Molecular Function:identical protein binding; protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity Biological Process: eye development; positive regulation of transcription of target genes involved in unfolded protein response; protein folding; regulation of transcription from RNA polymerase II promoter; response to stress; signal transduction; unfolded protein response; visual perception Disease: Achromatopsia 7 |
| NCBI Summary: | This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P18850 |
| NCBI GenInfo Identifier: | 66774203 |
| NCBI Gene ID: | 22926 |
| NCBI Accession: | P18850.3 |
| UniProt Secondary Accession: | P18850,O15139, Q5VW62, Q6IPB5, Q9UEC9, |
| UniProt Related Accession: | P18850 |
| Molecular Weight: | 74,585 Da |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-6 alpha |
| NCBI Synonym Full Names: | activating transcription factor 6 |
| NCBI Official Symbol: | ATF6 |
| NCBI Official Synonym Symbols: | ACHM7; ATF6A |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-6 alpha |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-6 alpha |
| UniProt Synonym Protein Names: | Activating transcription factor 6 alpha; ATF6-alpha |
| Protein Family: | Cyclic AMP-dependent transcription factor |
| UniProt Gene Name: | ATF6 |
| UniProt Entry Name: | ATF6A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATF6 antibody (CAB0202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded Rat ovary using ATF6 antibody (CAB0202) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human colon using ATF6 antibody (CAB0202) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Mouse kidney using ATF6 antibody (CAB0202) at dilution of 1:50 (40x lens). |
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