Name: Anti-ASIC3 Antibody (CAB10268)
Brand: Signal Transduction Antibodies 1
SKU: CAB10268
Availability: OutOfStock

Antibody Name:	Anti-ASIC3 Antibody
Antibody SKU:	CAB10268
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-320 of human ASIC3 (NP_064717.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse liver, Rat brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-320 of human ASIC3 (NP_064717.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ERVR YYRE FHHQ TALD ERES HRLI FPAV TLCN INPL RRSR LTPN DLHW AGSA LLGL DPAE HAAF LRAL GRPP APPG FMPS PTFD MAQL YARA GHSL DDML LDCR FRGQ PCGP ENFT TIFT RMGK CYTF NSGA DGAE LLTT TRGG MGNG LDIM LDVQ QEEY LPVW RDNE ETPF EVGI RVQI HSQE EPPI IDQL GLGV SPGY QTFV SCQQ QQLS FLPP PWGD CSSA SLNP NYEP EPSD PLGS PSPS PSPP YTLM GCRL ACE
Gene ID:	9311
Uniprot:	Q9UHC3
Cellular Location:	Cell membrane, Cytoplasm, Multi-pass membrane protein
Calculated MW:	44kDa/58kDa/60kDa
Observed MW:	59kDa

Synonyms:

ASIC3, ACCN3, DRASIC, SLNAC1, TNaC1

Background:

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 2 has been observed as proton-gated channels sensitive to gadolinium. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.
NCBI Summary:	This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 2 has been observed as proton-gated channels sensitive to gadolinium. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]
UniProt Code:	Q9UHC3
NCBI GenInfo Identifier:	82582992
NCBI Gene ID:	9311
NCBI Accession:	Q9UHC3.2
UniProt Secondary Accession:	Q9UHC3,O60263, O75906, Q59FN9, Q9UER8, Q9UHC4, B2R9V0
UniProt Related Accession:	Q9UHC3
Molecular Weight:	59kDa
NCBI Full Name:	Acid-sensing ion channel 3
NCBI Synonym Full Names:	acid sensing ion channel subunit 3
NCBI Official Symbol:	ASIC3
NCBI Official Synonym Symbols:	ACCN3; TNaC1; DRASIC; SLNAC1
NCBI Protein Information:	acid-sensing ion channel 3
UniProt Protein Name:	Acid-sensing ion channel 3
UniProt Synonym Protein Names:	Amiloride-sensitive cation channel 3; Neuronal amiloride-sensitive cation channel 3; Testis sodium channel 1; hTNaC1
Protein Family:	Acid-sensing ion channel
UniProt Gene Name:	ASIC3

Western blot analysis of extracts of various cell lines, using ASIC3 antibody (CAB10268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

ASIC3 Antibody (PACO01913)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)