Signal Transduction Antibodies 1

Anti-ASIC2 Antibody (CAB10496)

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SKU:
CAB10496
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-ASIC2 Antibody
Antibody SKU:CAB10496
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-400 of human ASIC2 (NP_001085.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, 293T, Mouse brain, Rat brain, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-400 of human ASIC2 (NP_001085.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NLQI PDPH LADP SVLE ALRQ KANF KHYK PKQF SMLE FLHR VGHD LKDM MLYC KFKG QECG HQDF TTVF TKYG KCYM FNSG EDGK PLLT TVKG GTGN GLEI MLDI QQDE YLPI WGET EETT FEAG VKVQ IHSQ SEPP FIQE LGFG VAPG FQTF VATQ EQRL TYLP PPWG ECRS SEMG LDFF PVYS ITAC RIDC ETRY IVEN CNCR MVHM PGDA PFCT PEQH KECA EPAL GLLA EKDS NYCL CRTP CNLT RYNK ELSM VKIP SKTS AKYL EKKF NKSE KYIS E
Gene ID:40
Uniprot:Q16515
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:57kDa/62kDa
Observed MW:58kDa
Synonyms:ASIC2, ACCN, ACCN1, ASIC2a, BNC1, BNaC1, MDEG, hBNaC1
Background:This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified.
UniProt Protein Function:ASIC2: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Channel, cation; Apoptosis; Transporter, ion channel; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: plasma membrane

Molecular Function:amiloride-sensitive sodium channel activity; protein binding

Biological Process: central nervous system development; monovalent inorganic cation transport; peripheral nervous system development; regulation of blood coagulation; regulation of gene expression; sensory perception of sour taste; synaptic transmission

NCBI Summary:This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Feb 2012]
UniProt Code:Q16515
NCBI GenInfo Identifier:2500840
NCBI Gene ID:40
NCBI Accession:Q16515.1
UniProt Secondary Accession:Q16515,Q13553, Q6DJU1, Q8N3E2, E9PBX2,
UniProt Related Accession:Q16515
Molecular Weight:62,887 Da
NCBI Full Name:Acid-sensing ion channel 2
NCBI Synonym Full Names:acid sensing ion channel subunit 2
NCBI Official Symbol:ASIC2  
NCBI Official Synonym Symbols:ACCN; BNC1; MDEG; ACCN1; BNaC1; ASIC2a; hBNaC1  
NCBI Protein Information:acid-sensing ion channel 2
UniProt Protein Name:Acid-sensing ion channel 2
UniProt Synonym Protein Names:Amiloride-sensitive brain sodium channel; Amiloride-sensitive cation channel 1, neuronal; Amiloride-sensitive cation channel neuronal 1; Brain sodium channel 1; BNC1; BNaC1; Mammalian degenerin homolog
Protein Family:Acid-sensing ion channel
UniProt Gene Name:ASIC2  
UniProt Entry Name:ASIC2_HUMAN
Anti-ASIC2 Antibody (CAB10496)Western blot analysis of extracts of various cell lines, using ASIC2 antibody (CAB10496) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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