Name: Anti-ASH2L Antibody (CAB14543)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB14543
Availability: OutOfStock

Antibody Name:	Anti-ASH2L Antibody
Antibody SKU:	CAB14543
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 81-280 of human ASH2L (NP_001098684.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	K-562, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 81-280 of human ASH2L (NP_001098684.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NLTW QSRT QDEH PKTM FSKD KDII PFID KYWE CMTT RQRP GKMT WPNN IVKT MSKE RDVF LVKE HPDP GSKD PEED YPKF GLLD QDLS NIGP AYDN QKQS SAVS TSGN LNGG IAAG SSGK GRGA KRKQ QDGG TTGT TKKA RSDP LFSA QRLP PHGY PLEH PFNK DGYR YILA EPDP HAPD PEKL ELDC WAGK PIPG DLYR
Gene ID:	9070
Uniprot:	Q9UBL3
Cellular Location:	Nucleus
Calculated MW:	56kDa/60kDa/68kDa
Observed MW:	69kDa

Synonyms:	ASH2L, ASH2, ASH2L1, ASH2L2, Bre2
Background:

UniProt Protein Function:	ASH2L: Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. Interacts with HCFC1. Core component of several methyltransferase-containing complexes including MLL1/MLL, ASCOM, MLL2/MLL3 and MLL3/MLL4. Each complex is at least composed of ASH2L, RBBP5, DPY30, WDR5, one or more specific histone methyltransferases (MLL, MLL2, MLL3 and MLL4), and the facultative components C16orf53/PA1, C17orf49, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, KAT8/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with DPY30 and RBBP5; the interaction is direct. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with SETD1A and SETD1B. Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription regulation; Methyltransferase Chromosomal Location of Human Ortholog: 8p11.2 Cellular Component: histone methyltransferase complex; nucleoplasm; nucleus Molecular Function:beta-catenin binding; DNA binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding Biological Process: histone H3-K4 methylation; positive regulation of cell proliferation; response to DNA damage stimulus; response to estrogen stimulus; transcription from RNA polymerase II promoter
UniProt Code:	Q9UBL3
NCBI GenInfo Identifier:	32141382
NCBI Gene ID:	9070
NCBI Accession:	Q9UBL3.1
UniProt Secondary Accession:	Q9UBL3,O60659, O60660, Q96B62, A8K7C3, D3DSW9,
UniProt Related Accession:	Q9UBL3
Molecular Weight:	60,208 Da
NCBI Full Name:	Set1/Ash2 histone methyltransferase complex subunit ASH2
NCBI Synonym Full Names:	ASH2 like histone lysine methyltransferase complex subunit
NCBI Official Symbol:	ASH2L
NCBI Official Synonym Symbols:	ASH2; Bre2; ASH2L1; ASH2L2
NCBI Protein Information:	set1/Ash2 histone methyltransferase complex subunit ASH2
UniProt Protein Name:	Set1/Ash2 histone methyltransferase complex subunit ASH2
UniProt Synonym Protein Names:	ASH2-like protein
UniProt Gene Name:	ASH2L
UniProt Entry Name:	ASH2L_HUMAN

Western blot analysis of extracts of various cell lines, using ASH2L antibody (CAB14543) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-ASH2L Antibody (CAB5392)

Anti-ASH2L Antibody (CAB17043)

Anti-ASH2L Antibody (CAB4892)

ASH2L Antibody (PACO07884)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)