Epigenetics & Nuclear Signaling Antibodies 1
Anti-ASCC2 Antibody (CAB13789)
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- SKU:
- CAB13789
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-ASCC2 Antibody |
| Antibody SKU: | CAB13789 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 488-757 of human ASCC2 (NP_115580.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, HepG2, A-431, HeLa, 293T, Mouse eye, Rat pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 488-757 of human ASCC2 (NP_115580.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LACL EYYH YDPE QVIN NILE ERLA PTLS QLDR NLDR EMKP DPTP LLTS RHNV FQND EFDV FSRD SVDL SRVH KGKS TRKE ENTR SLLN DKRA VAAQ RQRY EQYS VVVE EVPL QPGE SLPY HSVY YEDE YDDT YDGN QVGA NDAD SDDE LISR RPFT IPQV LRTK VPRE GQEE DDDD EEDD ADEE APKP DHFV QDPA VLRE KAEA RRMA FLAK KGYR HDSS TAVA GSPR GHGQ SRET TQER RKKE ANKA TRAN HNRR TMAD RKRS KGMI PS |
| Gene ID: | 84164 |
| Uniprot: | Q9H1I8 |
| Cellular Location: | |
| Calculated MW: | 41kDa/77kDa/86kDa |
| Observed MW: | 110kDa |
| Synonyms: | ASCC2, ASC1p100, p100 |
| Background: |
| UniProt Protein Function: | ASCC2: Enhances NF-kappa-B, SRF and AP1 transactivation. Belongs to the ASCC2 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 22q12.1 Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent |
| UniProt Code: | Q9H1I8 |
| NCBI GenInfo Identifier: | 92090990 |
| NCBI Gene ID: | 84164 |
| NCBI Accession: | Q9H1I8.3 |
| UniProt Secondary Accession: | Q9H1I8,Q4TT54, Q8TAZ0, Q9H711, Q9H9D6, B7Z8E0, F5H6J9 |
| UniProt Related Accession: | Q9H1I8 |
| Molecular Weight: | 77,369 Da |
| NCBI Full Name: | Activating signal cointegrator 1 complex subunit 2 |
| NCBI Synonym Full Names: | activating signal cointegrator 1 complex subunit 2 |
| NCBI Official Symbol: | ASCC2 |
| NCBI Official Synonym Symbols: | p100; ASC1p100 |
| NCBI Protein Information: | activating signal cointegrator 1 complex subunit 2; ASC-1 complex subunit P100; trip4 complex subunit p100 |
| UniProt Protein Name: | Activating signal cointegrator 1 complex subunit 2 |
| UniProt Synonym Protein Names: | ASC-1 complex subunit p100; Trip4 complex subunit p100 |
| Protein Family: | Activating signal cointegrator 1 complex |
| UniProt Gene Name: | ASCC2 |
| UniProt Entry Name: | ASCC2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ASCC2 antibody (CAB13789) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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