Cell Death Antibodies 1
Anti-ASAH2 Antibody (CAB12596)
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- SKU:
- CAB12596
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-ASAH2 Antibody |
| Antibody SKU: | CAB12596 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human ASAH2 (NP_001137446.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, Mouse kidney, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human ASAH2 (NP_001137446.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TATQ TSPV PLTP ESPL FQNF SGYH IGVG RADC TGQV ADIN LMGY GKSG QNAQ GILT RLYS RAFI MAEP DGSN RTVF VSID IGMV SQRL RLEV LNRL QSKY GSLY RRDN VILS GTHT HSGP AGYF QYTV FVIA SEGF SNQT FQHM VTGI LKSI DIAH TNMK PGKI FINK GNVD GVQI NRSP YSYL QNPQ SERA RYSS NTDK EMIV LKMV DLNG DDLG LISW FAIH PVSM NNSN HLVN SDNV GYAS YLLE QEKN KGYL PGQG P |
| Gene ID: | 56624 |
| Uniprot: | Q9NR71 |
| Cellular Location: | Cell membrane, Single-pass type II membrane protein |
| Calculated MW: | 81kDa/85kDa |
| Observed MW: | 85kDa |
| Synonyms: | ASAH2, BCDase, HNAC1, LCDase, N-CDase, NCDase |
| Background: |
| UniProt Protein Function: | ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - sphingolipid; EC 3.5.1.23; Hydrolase; Membrane protein, integral Chromosomal Location of Human Ortholog: 10q11.21 Cellular Component: mitochondrion Biological Process: ceramide metabolic process; signal transduction |
| NCBI Summary: | Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9NR71 |
| NCBI GenInfo Identifier: | 110832757 |
| NCBI Gene ID: | 56624 |
| NCBI Accession: | Q9NR71.2 |
| UniProt Secondary Accession: | Q9NR71,Q3KNU1, Q5SNT7, Q5SZP6, Q5SZP7, Q5T1D5, Q71ME6 |
| UniProt Related Accession: | Q9NR71 |
| Molecular Weight: | 81,718 Da |
| NCBI Full Name: | Neutral ceramidase |
| NCBI Synonym Full Names: | N-acylsphingosine amidohydrolase 2 |
| NCBI Official Symbol: | ASAH2 |
| NCBI Official Synonym Symbols: | HNAC1; BCDase; LCDase; NCDase; N-CDase |
| NCBI Protein Information: | neutral ceramidase |
| UniProt Protein Name: | Neutral ceramidase |
| UniProt Synonym Protein Names: | Acylsphingosine deacylase 2; BCDase; LCDase; hCD |
| Protein Family: | Neutral ceramidase |
| UniProt Gene Name: | ASAH2 |
| UniProt Entry Name: | ASAH2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ASAH2 antibody (CAB12596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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