Cell Biology Antibodies 2
Anti-ARRB2 Antibody (CAB1171)
- SKU:
- CAB1171
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ARRB2 Antibody |
| Antibody SKU: | CAB1171 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human ARRB2 (NP_004304.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, A-549 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human ARRB2 (NP_004304.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NLAS STIV KEGA NKEV LGIL VSYR VKVK LVVS RGGD VSVE LPFV LMHP KPHD HIPL PRPQ SAAP ETDV PVDT NLIE FDTN YATD DDIV FEDF ARLR LKGM K |
| Gene ID: | 409 |
| Uniprot: | P32121 |
| Cellular Location: | Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus, clathrin-coated pit |
| Calculated MW: | 44kDa/45kDa/46kDa/47kDa/48kDa |
| Observed MW: | 52kDa |
| Synonyms: | ARRB2, ARB2, ARR2, BARR2 |
| Background: | Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 17p13 Cellular Component: postsynaptic membrane; endocytic vesicle; basolateral plasma membrane; postsynaptic density; cytoplasm; plasma membrane; dendritic spine; coated pit; cytoplasmic vesicle; cytosol; nucleus Molecular Function:angiotensin receptor binding; protein domain specific binding; protein kinase B binding; type 2A serotonin receptor binding; follicle stimulating hormone receptor binding; alpha-1B adrenergic receptor binding; alpha-1A adrenergic receptor binding; protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; protein complex binding; mitogen-activated protein kinase binding; type 1 angiotensin receptor binding; protein complex scaffold; D1 dopamine receptor binding; receptor binding Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; follicle-stimulating hormone signaling pathway; positive regulation of receptor internalization; protein ubiquitination; negative regulation of caspase activity; adult walking behavior; protein transport; receptor internalization; transforming growth factor beta receptor signaling pathway; negative regulation of interleukin-6 production; detection of temperature stimulus involved in sensory perception of pain; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; platelet activation; negative regulation of interleukin-12 production; Notch signaling pathway; negative regulation of natural killer cell mediated cytotoxicity; positive regulation of synaptic transmission, dopaminergic; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; positive regulation of protein ubiquitination; negative regulation of toll-like receptor signaling pathway; negative regulation of protein ubiquitination; brain development; positive regulation of calcium ion transport; blood coagulation |
| NCBI Summary: | Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012] |
| UniProt Code: | P32121 |
| NCBI GenInfo Identifier: | 20141230 |
| NCBI Gene ID: | 409 |
| NCBI Accession: | P32121.2 |
| UniProt Related Accession: | P32121 |
| Molecular Weight: | ~ 52kDa |
| NCBI Full Name: | Beta-arrestin-2 |
| NCBI Synonym Full Names: | arrestin beta 2 |
| NCBI Official Symbol: | ARRB2 |
| NCBI Official Synonym Symbols: | ARB2; ARR2; BARR2 |
| NCBI Protein Information: | beta-arrestin-2 |
| UniProt Protein Name: | Beta-arrestin-2 |
| UniProt Synonym Protein Names: | Arrestin beta-2 |
| Protein Family: | Beta-arrestin |
| UniProt Gene Name: | ARRB2 |
| UniProt Entry Name: | ARRB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARRB2 antibody (CAB1171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of NIH/3T3 cells using ARRB2 Rabbit pAb (CAB1171) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using ARRB2 Rabbit pAb (CAB1171) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using ARRB2 Rabbit pAb (CAB1171) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Related Products
