Cell Biology Antibodies 12
Anti-ARPC5 Antibody (CAB8571)
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- SKU:
- CAB8571
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ARPC5 Antibody |
| Antibody SKU: | CAB8571 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human ARPC5 (NP_001257368.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse spleen, Mouse small intestine, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human ARPC5 (NP_001257368.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSKN TVSS ARFR KVDV DEYD ENKF VDEE DGGD GQAG PDEG EVDS CLRH SITG NMTA ALQA ALKN PPIN TKSQ AVKD RAGS IVLK VLIS FKAN DIEK AVQS LDKN GVDL LMKY IYKG FESP SDNS SAML LQWH EKAL AAGG VGSI VRVL TARK TV |
| Gene ID: | 10092 |
| Uniprot: | O15511 |
| Cellular Location: | Cell projection, Cytoplasm, cytoskeleton |
| Calculated MW: | 16kDa |
| Observed MW: | 18kDa |
| Synonyms: | ARPC5, ARC16, dJ127C7.3, p16-Arc |
| Background: | This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. |
| UniProt Protein Function: | ARPC5: Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Belongs to the ARPC5 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q25.3 Cellular Component: actin cytoskeleton; Arp2/3 protein complex; cytoplasm; cytosol; extracellular region; focal adhesion Molecular Function:actin filament binding; protein binding; structural constituent of cytoskeleton Biological Process: actin cytoskeleton organization and biogenesis; cell migration; cell motility; ephrin receptor signaling pathway; neutrophil degranulation |
| NCBI Summary: | This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
| UniProt Code: | O15511 |
| NCBI GenInfo Identifier: | 3121767 |
| NCBI Gene ID: | 10092 |
| NCBI Accession: | O15511.3 |
| UniProt Secondary Accession: | O15511,Q6PG42, A6NEC4, |
| UniProt Related Accession: | O15511 |
| Molecular Weight: | 16kDa |
| NCBI Full Name: | Actin-related protein 2/3 complex subunit 5 |
| NCBI Synonym Full Names: | actin related protein 2/3 complex subunit 5 |
| NCBI Official Symbol: | ARPC5 |
| NCBI Official Synonym Symbols: | ARC16; p16-Arc; dJ127C7.3 |
| NCBI Protein Information: | actin-related protein 2/3 complex subunit 5 |
| UniProt Protein Name: | Actin-related protein 2/3 complex subunit 5 |
| UniProt Synonym Protein Names: | Arp2/3 complex 16 kDa subunit; p16-ARC |
| UniProt Gene Name: | ARPC5 |
 | Western blot analysis of extracts of various cell lines, using ARPC5 antibody (CAB8571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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