Cell Biology Antibodies 11
Anti-ARPC3 Antibody (CAB7767)
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- SKU:
- CAB7767
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ARPC3 Antibody |
| Antibody SKU: | CAB7767 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human ARPC3 (NP_001265485.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, SKOV3, HeLa, SH-SY5Y, Mouse spleen, Mouse lung, Rat ovary |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human ARPC3 (NP_001265485.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPAY HSSL MDPD TKLI GNMA LLPI RSQF KGPA PRET KDTD IVDE AIYY FKAN VFFK NYEI KNEA DRTL IYIT LYIS ECLK KLQK CNSK SQGE KEMY TLGI TNFP IPGE PGFP LNAI YAKP ANKQ EDEV MRAY LQQL RQET GLRL CEKV FDPQ NDKP SKWW TCFV KRQF MNKS LSGP GQ |
| Gene ID: | 10094 |
| Uniprot: | O15145 |
| Cellular Location: | Cell projection, Cytoplasm, cytoskeleton |
| Calculated MW: | 20kDa |
| Observed MW: | 21kDa |
| Synonyms: | ARPC3, ARC21, p21-Arc |
| Background: | This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | ARPC3: one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 12q24.11 Cellular Component: Arp2/3 protein complex; focal adhesion; membrane; lamellipodium; cytosol; actin cytoskeleton Molecular Function:actin filament binding; protein binding; structural constituent of cytoskeleton Biological Process: axon guidance; ephrin receptor signaling pathway; innate immune response; cell motility |
| NCBI Summary: | This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013] |
| UniProt Code: | O15145 |
| NCBI GenInfo Identifier: | 3121765 |
| NCBI Gene ID: | 10094 |
| NCBI Accession: | O15145.3 |
| UniProt Secondary Accession: | O15145,O00554, |
| UniProt Related Accession: | O15145 |
| Molecular Weight: | 178 |
| NCBI Full Name: | Actin-related protein 2/3 complex subunit 3 |
| NCBI Synonym Full Names: | actin related protein 2/3 complex, subunit 3, 21kDa |
| NCBI Official Symbol: | ARPC3 |
| NCBI Official Synonym Symbols: | ARC21; p21-Arc |
| NCBI Protein Information: | actin-related protein 2/3 complex subunit 3; arp2/3 complex 21 kDa subunit; ARP2/3 protein complex subunit p21 |
| UniProt Protein Name: | Actin-related protein 2/3 complex subunit 3 |
| UniProt Synonym Protein Names: | Arp2/3 complex 21 kDa subunit; p21-ARC |
| Protein Family: | Actin-related protein 2/3 complex |
| UniProt Gene Name: | ARPC3 |
| UniProt Entry Name: | ARPC3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARPC3 Antibody (CAB7767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of A549 cells using ARPC3 antibody (CAB7767). Blue: DAPI for nuclear staining. |
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