Antibody Name:	Anti-ARL4D Antibody
Antibody SKU:	CAB16761
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 92-201 of human ARL4D (NP_001652.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 92-201 of human ARL4D (NP_001652.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RRTD GLVF VVDA AEAE RLEE AKVE LHRI SRAS DNQG VPVL VLAN KQDQ PGAL SAAE VEKR LAVR ELAA ATLT HVQG CSAV DGLG LQQG LERL YEMI LKRK KAAR GGKK RR
Gene ID:	379
Uniprot:	P49703
Cellular Location:	Golgi apparatus, Lipid-anchor, Membrane
Calculated MW:
Observed MW:	22kDa

Synonyms:

ARL4D, ARF4L, ARL6

Background:

ADP-ribosylation factor 4D is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4D is closely similar to ARL4A and ARL4C and each has a nuclear localization signal and an unusually high guanine nucleotide exchange rate. This protein may play a role in membrane-associated intracellular trafficking. Mutations in this gene have been associated with Bardet-Biedl syndrome (BBS).

UniProt Protein Function:	ARL4D: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:	Protein type:G protein; Nucleolus; G protein, monomeric; G protein, monomeric, ARF Chromosomal Location of Human Ortholog: 17q21.31 Cellular Component: cytoplasm; nucleolus; plasma membrane Molecular Function:GTPase activity; protein binding; GTP binding Biological Process: metabolic process; small GTPase mediated signal transduction; protein secretion
NCBI Summary:	ADP-ribosylation factor 4D is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4D is closely similar to ARL4A and ARL4C and each has a nuclear localization signal and an unusually high guanine nucleotide exchange rate. This protein may play a role in membrane-associated intracellular trafficking. Mutations in this gene have been associated with Bardet-Biedl syndrome (BBS). [provided by RefSeq, Jul 2008]
UniProt Code:	P49703
NCBI GenInfo Identifier:	41327767
NCBI Gene ID:	379
NCBI Accession:	NP_001652.2
UniProt Secondary Accession:	P49703,B2RC59, D3DX43,
UniProt Related Accession:	P49703
Molecular Weight:	22,156 Da
NCBI Full Name:	ADP-ribosylation factor-like protein 4D
NCBI Synonym Full Names:	ADP-ribosylation factor-like 4D
NCBI Official Symbol:	ARL4D
NCBI Official Synonym Symbols:	ARL6; ARF4L
NCBI Protein Information:	ADP-ribosylation factor-like protein 4D; ADP-ribosylation factor-like 6; ADP-ribosylation factor-like protein 4L
UniProt Protein Name:	ADP-ribosylation factor-like protein 4D
UniProt Synonym Protein Names:	ADP-ribosylation factor-like protein 4L
Protein Family:	ADP-ribosylation factor-like protein
UniProt Gene Name:	ARL4D
UniProt Entry Name:	ARL4D_HUMAN

	Western blot analysis of extracts of various cell lines, using ARL4D antibody (CAB16761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using ARL4D antibody (CAB16761) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using ARL4D antibody (CAB16761) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using ARL4D antibody (CAB16761) at dilution of 1:100 (40x lens).