Signal Transduction Antibodies 2
Anti-ARHGEF5 Antibody (CAB4196)
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- SKU:
- CAB4196
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-ARHGEF5 Antibody |
| Antibody SKU: | CAB4196 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1248-1597 of human ARHGEF5 (NP_005426.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, A-549, HepG2, 293T, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1248-1597 of human ARHGEF5 (NP_005426.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QVCD VVLN HAPD FRRV YLPY VTNQ TYQE RTFQ SLMN SNSN FREV LEKL ESDP VCQR LSLK SFLI LPFQ RITR LKLL LQNI LKRT QPGS SEEA EATK AHHA LEQL IRDC NNNV QSMR RTEE LIYL SQKI EFEC KIFP LISQ SRWL VKSG ELTA LEFS ASPG LRRK LNTR PVHL HLFN DCLL LSRP REGS RFLV FDHA PFSS IRGE KCEM KLHG PHKN LFRL FLRQ NTQG AQAE FLFR TETQ SEKL RWIS ALAM PREE LDLL ECYN SPQV QCLR AYKP REND ELAL EKAD VVMV TQQS SDGW LEGV RLSD GERG WFPV QQVE FISN PEVR AQNL KEAH RVKT AKLQ LVEQ QA |
| Gene ID: | 7984 |
| Uniprot: | Q12774 |
| Cellular Location: | |
| Calculated MW: | 60kDa/176kDa |
| Observed MW: | 176kDa |
| Synonyms: | ARHGEF5, GEF5, P60, TIM, TIM1 |
| Background: | Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. |
| UniProt Protein Function: | ARHGEF5: 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GEFs; GEFs, Rac/Rho; Motility/polarity/chemotaxis; Oncoprotein Chromosomal Location of Human Ortholog: 7q35 Cellular Component: cytoplasm; nucleoplasm; nucleus; plasma membrane Molecular Function:GTP binding; guanyl-nucleotide exchange factor activity; protein binding Biological Process: positive regulation of JNK activity; positive regulation of stress fiber formation; positive regulation of transcription factor activity; regulation of actin cytoskeleton organization and biogenesis; regulation of cytoskeleton organization and biogenesis; regulation of GTPase activity |
| NCBI Summary: | Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12774 |
| NCBI GenInfo Identifier: | 290457679 |
| NCBI Gene ID: | 7984 |
| NCBI Accession: | Q12774.3 |
| UniProt Secondary Accession: | Q12774,Q6ZML7, A6NNJ2, |
| UniProt Related Accession: | Q12774 |
| Molecular Weight: | 60,056 Da |
| NCBI Full Name: | Rho guanine nucleotide exchange factor 5 |
| NCBI Synonym Full Names: | Rho guanine nucleotide exchange factor 5 |
| NCBI Official Symbol: | ARHGEF5 |
| NCBI Official Synonym Symbols: | P60; TIM; GEF5; TIM1 |
| NCBI Protein Information: | rho guanine nucleotide exchange factor 5 |
| UniProt Protein Name: | Rho guanine nucleotide exchange factor 5 |
| UniProt Synonym Protein Names: | Ephexin-3; Guanine nucleotide regulatory protein TIM; Oncogene TIM; Transforming immortalized mammary oncogene; p60 TIM |
| Protein Family: | Rho guanine nucleotide exchange factor |
| UniProt Gene Name: | ARHGEF5 |
| UniProt Entry Name: | ARHG5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARHGEF5 antibody (CAB4196) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded mouse liver using ARHGEF5 antibody (CAB4196) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using ARHGEF5 antibody (CAB4196) at dilution of 1:100 (40x lens). |
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