Antibody Name:	Anti-ARHGEF3 Antibody
Antibody SKU:	CAB8490
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 287-526 of human ARHGEF3 (NP_062455.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 287-526 of human ARHGEF3 (NP_062455.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LEEA INII QGIV AEIN TKTG ESEC RYYK ERLL YLEE GQKD SLID SSRV LCCH GELK NNRG VKLH VFLF QEVL VITR AVTH NEQL CYQL YRQP IPVK DLLL EDLQ DGEV RLGG SLRG AFSN NERI KNFF RVSF KNGS QSQT HSLQ ANDT FNKQ QWLN CIRQ AKET VLCA AGQA GVLD SEGS FLNP TTGS RELQ GETK LEQM DQSD SESD CSMD TSEV SLDC ERME QTDS SCGN SRHG ESNV
Gene ID:	50650
Uniprot:	Q9NR81
Cellular Location:	Cytoplasm
Calculated MW:	36kDa/59kDa/60kDa/63kDa
Observed MW:	60kDa

Synonyms:

ARHGEF3, GEF3, STA3, XPLN

Background:

Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ARHGEF3: Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; GAPs, Rac/Rho; GAPs Chromosomal Location of Human Ortholog: 3p14.3 Cellular Component: cytosol; intracellular Molecular Function:protein binding; Rho guanyl-nucleotide exchange factor activity Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of GTPase activity; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; small GTPase mediated signal transduction
NCBI Summary:	Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NR81
NCBI GenInfo Identifier:	52782760
NCBI Gene ID:	50650
NCBI Accession:	Q9NR81.1
UniProt Secondary Accession:	Q9NR81,Q4FZB6, Q4QQI5, Q4QQQ0, Q59F00, Q6NUN3, Q7Z4U2 Q7Z5T2, Q9H7T4, A8K5U7,
UniProt Related Accession:	Q9NR81
Molecular Weight:	36,981 Da
NCBI Full Name:	Rho guanine nucleotide exchange factor 3
NCBI Synonym Full Names:	Rho guanine nucleotide exchange factor 3
NCBI Official Symbol:	ARHGEF3
NCBI Official Synonym Symbols:	GEF3; STA3; XPLN
NCBI Protein Information:	rho guanine nucleotide exchange factor 3
UniProt Protein Name:	Rho guanine nucleotide exchange factor 3
UniProt Synonym Protein Names:	Exchange factor found in platelets and leukemic and neuronal tissues; XPLN
Protein Family:	Rho guanine nucleotide exchange factor
UniProt Gene Name:	ARHGEF3
UniProt Entry Name:	ARHG3_HUMAN

	Western blot analysis of extracts of various cell lines, using ARHGEF3 antibody (CAB8490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse brain using ARHGEF3 antibody (CAB8490) at dilution of 1:100 (40x lens).