Antibody Name:	ARHGEF11 Rabbit Polyclonal Antibody
Antibody SKU:	CAB7954
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human ARHGEF11

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse brain

Immunogen:	A synthetic peptide of human ARHGEF11
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	9826
Uniprot:	O15085
Cellular Location:	Cytoplasm, Membrane
Calculated MW:	167kDa/172kDa
Observed MW:	168kDa

Synonyms:

ARHGEF11, GTRAP48, PDZ-RHOGEF

Background:

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described.

UniProt Protein Function:	ARHGEF11: May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; GEFs, Rac/Rho; GEFs Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytoplasm; cytosol; intracellular Molecular Function:G-protein-coupled receptor binding; guanyl-nucleotide exchange factor activity; protein binding; Rho guanyl-nucleotide exchange factor activity Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; regulation of small GTPase mediated signal transduction; Rho protein signal transduction
NCBI Summary:	Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	O15085
NCBI GenInfo Identifier:	34395516
NCBI Gene ID:	9826
NCBI Accession:	O15085.1
UniProt Secondary Accession:	O15085,Q5VY40, Q6PFW2, D3DVD0,
UniProt Related Accession:	O15085
Molecular Weight:	172,244 Da
NCBI Full Name:	Rho guanine nucleotide exchange factor 11
NCBI Synonym Full Names:	Rho guanine nucleotide exchange factor 11
NCBI Official Symbol:	ARHGEF11
NCBI Official Synonym Symbols:	GTRAP48; PDZ-RHOGEF
NCBI Protein Information:	rho guanine nucleotide exchange factor 11
UniProt Protein Name:	Rho guanine nucleotide exchange factor 11
UniProt Synonym Protein Names:	PDZ-RhoGEF
Protein Family:	Rho guanine nucleotide exchange factor
UniProt Gene Name:	ARHGEF11
UniProt Entry Name:	ARHGB_HUMAN

	Western blot analysis of extracts of mouse brain, using ARHGEF11 antibody (CAB7954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human prostate cancer using ARHGEF11 antibody (CAB7954) (40x lens).
	Immunohistochemistry of paraffin-embedded human lung using ARHGEF11 antibody (CAB7954) (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using ARHGEF11 antibody (CAB7954) (40x lens).