Cell Biology Antibodies 8

Anti-ARHGDIB Antibody (CAB3740)

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SKU:
CAB3740
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ARHGDIB Antibody
Antibody SKU:CAB3740
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human ARHGDIB (NP_001166.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Raji, HL-60, HT-1080, Mouse brain, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human ARHGDIB (NP_001166.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTEK APEP HVEE DDDD ELDS KLNY KPPP QKSL KELQ EMDK DDES LIKY KKTL LGDG PVVT DPKA PNVV VTRL TLVC ESAP GPIT MDLT GDLE ALKK ETIV LKEG SEYR VKIH FKVN RDIV SGLK YVQH TYRT GVKV DKAT FMVG SYGP RPEE YEFL TPVE EAPK GMLA RGTY HNKS FFTD DDKQ DHLS WEWN LSIK KEWT E
Gene ID:397
Uniprot:P52566
Cellular Location:Cytoplasm
Calculated MW:22kDa
Observed MW:23kDa
Synonyms:ARHGDIB, D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2
Background:
UniProt Protein Function:RhoGDI beta: regulates the GDP/GTP exchange reaction of Rho proteins by inhibiting the dissociation of GDP from them, keeping these small GTPases in an inactive state. The GDIs are made up of two domains: a flexible N-terminal domain of about 70 amino acid residues and a folded 134-residue C-terminal domain. Regulates the GTP/GDP cycling of glucose-responsive small GTPases. Its phosphorylation on Y156, S101, and S174 mediates the cycling of Cdc42 and Rac1 to regulate second-phase insulin secretion. Interacts with WNK1.
UniProt Protein Details:

Protein type:G protein regulator, misc.; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: cytoskeleton; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol

Molecular Function:Rho GDP-dissociation inhibitor activity; GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; regulation of catalytic activity; small GTPase mediated signal transduction; multicellular organismal development; immune response; negative regulation of cell adhesion; cell motility; actin cytoskeleton organization and biogenesis; Rho protein signal transduction; positive regulation of GTPase activity

NCBI Summary:Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).[supplied by OMIM, Dec 2010]
UniProt Code:P52566
NCBI GenInfo Identifier:1707893
NCBI Gene ID:397
NCBI Accession:P52566.3
UniProt Secondary Accession:P52566,B5BU79,
UniProt Related Accession:P52566
Molecular Weight:201
NCBI Full Name:Rho GDP-dissociation inhibitor 2
NCBI Synonym Full Names:Rho GDP dissociation inhibitor (GDI) beta
NCBI Official Symbol:ARHGDIB  
NCBI Official Synonym Symbols:D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2  
NCBI Protein Information:rho GDP-dissociation inhibitor 2; Rho GDI 2; rho-GDI beta
UniProt Protein Name:Rho GDP-dissociation inhibitor 2
UniProt Synonym Protein Names:Ly-GDI; Rho-GDI beta
Protein Family:Rho GDP-dissociation inhibitor
UniProt Gene Name:ARHGDIB  
UniProt Entry Name:GDIR2_HUMAN
Anti-ARHGDIB Antibody (CAB3740)Western blot analysis of extracts of various cell lines, using ARHGDIB antibody (CAB3740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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