Name: Anti-ARHGAP22 Antibody (CAB16566)
Brand: Cardiovascular Antibodies
SKU: CAB16566
Availability: OutOfStock

Antibody Name:	Anti-ARHGAP22 Antibody
Antibody SKU:	CAB16566
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 510-650 of human ARHGAP22 (NP_001242953.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse kidney, Mouse lung, Rat kidney, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 510-650 of human ARHGAP22 (NP_001242953.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NVPA PGLV PGIP SVAS MAWS GASS SESS VGGS LSSC TACR ASDS SARS SLHT DWAL EPSP LPSS SEDP KSLD LDHS MDEA GAGA SNSE PSEP DSPT REHA RRSE ALQG LVTE LRAE LCRQ RTEY ERSV KRIE EGSA DLRK R
Gene ID:	58504
Uniprot:	Q7Z5H3
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	52kDa/66kDa/76kDa/77kDa/78kDa
Observed MW:	77kDa

Synonyms:

ARHGAP22, RhoGAP2, RhoGap22

Background:

This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ARHGAP22: Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GAPs; GAPs, Rac/Rho Chromosomal Location of Human Ortholog: 10q11.22 Cellular Component: cytosol; focal adhesion Molecular Function:GTPase activator activity; protein binding Biological Process: regulation of small GTPase mediated signal transduction
NCBI Summary:	This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q7Z5H3
NCBI GenInfo Identifier:	74750129
NCBI Gene ID:	58504
NCBI Accession:	Q7Z5H3.1
UniProt Secondary Accession:	Q7Z5H3,O00152, Q6ZSB0, A0AVP7, A5YM75, B4DED8, B9EGA0 C9JDM2,
UniProt Related Accession:	Q7Z5H3
Molecular Weight:	77,457 Da
NCBI Full Name:	Rho GTPase-activating protein 22
NCBI Synonym Full Names:	Rho GTPase activating protein 22
NCBI Official Symbol:	ARHGAP22
NCBI Official Synonym Symbols:	RhoGAP2; RhoGap22
NCBI Protein Information:	rho GTPase-activating protein 22
UniProt Protein Name:	Rho GTPase-activating protein 22
UniProt Synonym Protein Names:	Rho-type GTPase-activating protein 22
Protein Family:	Rho GTPase-activating protein
UniProt Gene Name:	ARHGAP22
UniProt Entry Name:	RHG22_HUMAN

Western blot analysis of extracts of various cell lines, using ARHGAP22 antibody (CAB16566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.

Antibodies

ARHGAP22 Antibody (PACO04860)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)