Cell Biology Antibodies 12

Anti-ARFGAP3 Antibody (CAB8204)

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SKU:
CAB8204
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:ARFGAP3 Rabbit Polyclonal Antibody
Antibody SKU:CAB8204
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 217-516 of human ARFGAP3 (NP_055385.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:HepG2, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 217-516 of human ARFGAP3 (NP_055385.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KPNQ AKKG LGAK KGSL GAQK LANT CFNE IEKQ AQAA DKMK EQED LAKV VSKE ESIV SSLR LAYK DLEI QMKK DEKM NISG KKNV DSDR LGMG FGNC RSVI SHSV TSDM QTIE QESP IMAK PRKK YNDD SDDS YFTS SSSY FDEP VELR SSSF SSWD DSSD SYWK KETS KDTE TVLK TTGY SDRP TARR KPDY EPVE NTDE AQKK FGNV KAIS SDMY FGRQ SQAD YETR ARLE RLSA SSSI SSAD LFEE PRKQ PAGN YSLS SVLP NAPD MAQF KQGV RSVA GKLS VFAN GVVT SIQD RYGS
Gene ID:26286
Uniprot:Q9NP61
Cellular Location:Cytoplasm, Cytoplasmic side, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:51kDa/56kDa
Observed MW:57kDa
Synonyms:ARFGAP3, ARFGAP1
Background:The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.
UniProt Protein Function:GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
NCBI Summary:The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]
UniProt Code:Q9NP61
NCBI GenInfo Identifier:21263420
NCBI Gene ID:26286
NCBI Accession:Q9NP61.1
UniProt Secondary Accession:Q9NP61,Q9BSC6, Q9H9J0, Q9NT10, Q9NUP5, Q9Y4V3, Q9Y4V4 E9PB03,
UniProt Related Accession:Q9NP61
Molecular Weight:51,990 Da
NCBI Full Name:ADP-ribosylation factor GTPase-activating protein 3
NCBI Synonym Full Names:ADP ribosylation factor GTPase activating protein 3
NCBI Official Symbol:ARFGAP3  
NCBI Official Synonym Symbols:ARFGAP1  
NCBI Protein Information:ADP-ribosylation factor GTPase-activating protein 3
UniProt Protein Name:ADP-ribosylation factor GTPase-activating protein 3
Protein Family:ADP-ribosylation factor GTPase-activating protein
UniProt Gene Name:ARFGAP3  
UniProt Entry Name:ARFG3_HUMAN
Anti-ARFGAP3 Polyclonal Antibody (CAB8204)Western blot analysis of extracts of various cell lines, using ARFGAP3 antibody (CAB8204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ARFGAP3 Polyclonal Antibody (CAB8204)Immunohistochemistry of paraffin-embedded mouse stomach using ARFGAP3 antibody (CAB8204) at dilution of 1:100 (40x lens).
Anti-ARFGAP3 Polyclonal Antibody (CAB8204)Immunohistochemistry of paraffin-embedded human liver cancer using ARFGAP3 antibody (CAB8204) at dilution of 1:100 (40x lens).
Anti-ARFGAP3 Polyclonal Antibody (CAB8204)Immunohistochemistry of paraffin-embedded human esophagus using ARFGAP3 antibody (CAB8204) at dilution of 1:100 (40x lens).
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