Antibody Name:	Anti-ARFGAP1 Antibody
Antibody SKU:	CAB12595
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARFGAP1 (NP_001268411.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, MCF7, Mouse ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARFGAP1 (NP_001268411.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASP RTRK VLKE VRVQ DENN VCFE CGAF NPQW VSVT YGIW ICLE CSGR HRGL GVHL SFVR SVTM DKWK DIEL EKMK AGGN AKFR EFLE SQED YDPC WSLQ EKYN SRAA ALFR DKVV ALAE GREW SLES SPAQ NWTP PQPR TLPS MVHR VSGQ PQSV TASS DKAF EDWL NDDL GSYQ GAQG NRYV GFGN TPPP QKKE DDFL NNAM SSLY SGWS SFTT GASR
Gene ID:	55738
Uniprot:	Q8N6T3
Cellular Location:	Cytoplasm, Golgi apparatus
Calculated MW:	31kDa/39kDa/44kDa/45kDa
Observed MW:	42-45kDa

Synonyms:

ARFGAP1, ARF1GAP, HRIHFB2281

Background:

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ARF GAP1: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; GAPs; GAPs, ARF Chromosomal Location of Human Ortholog: 20q13.33 Cellular Component: synapse; cytosol Molecular Function:protein binding; zinc ion binding; GTPase activator activity Biological Process: protein transport; unfolded protein response, activation of signaling protein activity; COPI coating of Golgi vesicle; cellular protein metabolic process; unfolded protein response; retrograde vesicle-mediated transport, Golgi to ER; positive regulation of GTPase activity
NCBI Summary:	The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:	Q8N6T3
NCBI GenInfo Identifier:	27923731
NCBI Gene ID:	55738
NCBI Accession:	Q8N6T3.2
UniProt Secondary Accession:	Q8N6T3,Q6PK71, Q96KC4, Q96T02, Q9NSU3, Q9NVF6, Q9UIL0 B7Z3U0, B7Z8H8, B7ZBI3, E1P5I9, E7EV62,
UniProt Related Accession:	Q8N6T3
Molecular Weight:	406
NCBI Full Name:	ADP-ribosylation factor GTPase-activating protein 1
NCBI Synonym Full Names:	ADP-ribosylation factor GTPase activating protein 1
NCBI Official Symbol:	ARFGAP1
NCBI Official Synonym Symbols:	ARF1GAP; HRIHFB2281
NCBI Protein Information:	ADP-ribosylation factor GTPase-activating protein 1; ARF1-directed GTPase-activating protein
UniProt Protein Name:	ADP-ribosylation factor GTPase-activating protein 1
UniProt Synonym Protein Names:	ADP-ribosylation factor 1 GTPase-activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein
Protein Family:	ADP-ribosylation factor GTPase-activating protein
UniProt Gene Name:	ARFGAP1
UniProt Entry Name:	ARFG1_HUMAN

	Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (CAB12595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded rat brain using ARFGAP1 antibody (CAB12595) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using ARFGAP1 antibody (CAB12595) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using ARFGAP1 antibody (CAB12595). Blue: DAPI for nuclear staining.