Cell Cycle Antibodies 1
Anti-ARF6 Antibody (CAB0988)
- SKU:
- CAB0988
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-ARF6 Antibody |
| Antibody SKU: | CAB0988 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human ARF6 (NP_001654.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, DU145, Mouse brain, Rat brain, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human ARF6 (NP_001654.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGKV LSKI FGNK EMRI LMLG LDAA GKTT ILYK LKLG QSVT TIPT VGFN VETV TYKN VKFN VWDV GGQD KIRP LWRH YYTG TQGL IFVV DCAD RDRI DEAR QELH RIIN DREM RDAI ILIF ANKQ DLPD AMKP HEIQ EKLG LTRI RDRN WYVQ PSCA TSGD GLYE GLTW LTSN YKS |
| Gene ID: | 382 |
| Uniprot: | P62330 |
| Cellular Location: | Cell membrane, Cell projection, Cleavage furrow, Cytoplasm, Endosome membrane, Golgi apparatus, Lipid-anchor, Midbody, Recycling endosome membrane, filopodium membrane |
| Calculated MW: | 20kDa |
| Observed MW: | 19kDa |
| Synonyms: | ARF6 |
| Background: | This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. |
| UniProt Protein Function: | GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed:11266366, PubMed:21170023, PubMed:16737952, PubMed:7589240, PubMed:18400762). Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed:14978216). Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1. |
| NCBI Summary: | This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62330 |
| NCBI GenInfo Identifier: | 51316984 |
| NCBI Gene ID: | 382 |
| NCBI Accession: | P62330.2 |
| UniProt Secondary Accession: | P62330,P26438, Q6FGZ2, |
| UniProt Related Accession: | P62330 |
| Molecular Weight: | 20,082 Da |
| NCBI Full Name: | ADP-ribosylation factor 6 |
| NCBI Synonym Full Names: | ADP ribosylation factor 6 |
| NCBI Official Symbol: | ARF6 |
| NCBI Protein Information: | ADP-ribosylation factor 6 |
| UniProt Protein Name: | ADP-ribosylation factor 6 |
| Protein Family: | Auxin response factor |
| UniProt Gene Name: | ARF6 |
 | Western blot analysis of extracts of various cell lines, using ARF6 antibody (CAB0988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
 | Immunofluorescence analysis of HeLa cells using ARF6 antibody (CAB0988) at dilution of 1:100. Blue: DAPI for nuclear staining. |
View AllClose
Related Products
Human ARF6 Recombinant Protein (His tag)
OverviewProduct Name:Human ARF6 Recombinant Protein (His tag)Product Code:RPES5701Size:20µgSpecies:HumanExpress
