Signal Transduction Antibodies 2

Anti-AQP3 Antibody (CAB2838)

(No reviews yet) Write a Review
SKU:
CAB2838
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-AQP3 Antibody
Antibody SKU:CAB2838
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 178-292 of human AQP3 (NP_004916.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 178-292 of human AQP3 (NP_004916.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IVDP YNNP VPRG LEAF TVGL VVLV IGTS MGFN SGYA VNPA RDFG PRLF TALA GWGS AVFT TGQH WWWV PIVS PLLG SIAG VFVY QLMI GCHL EQPP PSNE EENV KLAH VKHK EQI
Gene ID:360
Uniprot:Q92482
Cellular Location:Basolateral cell membrane, Multi-pass membrane protein
Calculated MW:29kDa/31kDa
Observed MW:31kDa
Synonyms:AQP3, AQP-3, GIL
Background:This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:AQP3: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Belongs to the MIP/aquaporin (TC 1.A.8) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transporter; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: integral to plasma membrane; basolateral plasma membrane; cytoplasm; integral to membrane; plasma membrane; intercellular junction

Molecular Function:transporter activity; urea transmembrane transporter activity; water channel activity; glycerol channel activity

Biological Process: glycerol transport; positive regulation of immune system process; cellular water homeostasis; response to retinoic acid; water transport; excretion; odontogenesis; response to vitamin D; transport; renal water homeostasis; response to calcium ion; regulation of keratinocyte differentiation; transmembrane transport

Disease: Gil Blood Group

NCBI Summary:This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]
UniProt Code:Q92482
NCBI GenInfo Identifier:2497938
NCBI Gene ID:360
NCBI Accession:Q92482.2
UniProt Secondary Accession:Q92482,O00108, Q6FGT2, Q6FGW6, A8K843, B2RE16, D3DRL3
UniProt Related Accession:Q92482
Molecular Weight:29,987 Da
NCBI Full Name:Aquaporin-3
NCBI Synonym Full Names:aquaporin 3 (Gill blood group)
NCBI Official Symbol:AQP3  
NCBI Official Synonym Symbols:GIL; AQP-3  
NCBI Protein Information:aquaporin-3; aquaglyceroporin-3; aquaporin 3 (GIL blood group)
UniProt Protein Name:Aquaporin-3
UniProt Synonym Protein Names:Aquaglyceroporin-3
Protein Family:Aquaporin
UniProt Gene Name:AQP3  
UniProt Entry Name:AQP3_HUMAN
Anti-AQP3 Antibody (CAB2838)Western blot analysis of extracts of various cell lines, using AQP3 antibody (CAB2838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5min.
Anti-AQP3 Antibody (CAB2838)Immunohistochemistry of paraffin-embedded rat kidney using AQP3 Rabbit pAb (CAB2838) at dilution of 1:50 (40x lens).
Anti-AQP3 Antibody (CAB2838)Immunohistochemistry of paraffin-embedded mouse kidney using AQP3 Rabbit pAb (CAB2838) at dilution of 1:50 (40x lens).
Anti-AQP3 Antibody (CAB2838)Immunofluorescence analysis of rat kidney using AQP3 antibody (CAB2838) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-AQP3 Antibody (CAB2838)Immunofluorescence analysis of mouse kidney using AQP3 antibody (CAB2838) at dilution of 1:100. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products