Cell Cycle Antibodies 1

Anti-APPL2 Antibody (CAB14590)

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SKU:
CAB14590
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-APPL2 Antibody
Antibody SKU:CAB14590
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human APPL2 (NP_060641.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, HT-29, Mouse heart, Rat pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human APPL2 (NP_060641.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPAV DKLL LEEA LQDS PQTR SLLS VFEE DAGT LTDY TNQL LQAM QRVY GAQN EMCL ATQQ LSKQ LLAY EKQN FALG KGDE EVIS TLHY FSKV VDEL NLLH TELA KQLA DTMV LPII QFRE KDLT EVST LKDL FGLA SNEH DLSM AKYS RLPK KKEN EKVK TEVG KEVA AARR KQHL SSLQ YYCA LNAL QYRK QMAM MEPM IGFA HGQI NFFK KGAE MFSK RMDS FLSS VADM VQSI QVEL EAEA EKMR VSQQ ELLS VDES VYTP DSDV AAPQ INRN LIQK AGYL NLRN KTGL VTTT WERL
Gene ID:55198
Uniprot:Q8NEU8
Cellular Location:Early endosome membrane, Nucleus, Peripheral membrane protein
Calculated MW:69kDa/74kDa/75kDa
Observed MW:74kDa
Synonyms:APPL2, DIP13B
Background:The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome.
UniProt Protein Function:APPL2: a Rab5 effector protein that resides on a subpopulation of endosomes. Required for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction. Its function requires Rab5 binding. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF. Binds to subunits of the the nucleosome remodeling and deacetylase (NuRD) complex, an abundant and widely expressed deacetylase complex. The NURD complex contains both histone deacetylation and chromatin remodeling ATPase activities. Contains a PH domain and a phosphotyrosine interaction domain (PID) domain that has a structure similar to the insulin receptor substrate-1 PTB domain.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: cytoplasm; endosome membrane; nucleus; NuRD complex

Molecular Function:protein binding

Biological Process: cell proliferation; signal transduction

NCBI Summary:The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome. [provided by RefSeq, Oct 2011]
UniProt Code:Q8NEU8
NCBI GenInfo Identifier:160419148
NCBI Gene ID:55198
NCBI Accession:Q8NEU8.3
UniProt Secondary Accession:Q8NEU8,Q8N4R7, Q9NVL2, B7Z411, B7Z4B0, F5GZG0, F8W1P5
UniProt Related Accession:Q8NEU8
Molecular Weight:75,184 Da
NCBI Full Name:DCC-interacting protein 13-beta
NCBI Synonym Full Names:adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2
NCBI Official Symbol:APPL2  
NCBI Official Synonym Symbols:DIP13B  
NCBI Protein Information:DCC-interacting protein 13-beta
UniProt Protein Name:DCC-interacting protein 13-beta
UniProt Synonym Protein Names:Adapter protein containing PH domain, PTB domain and leucine zipper motif 2
Protein Family:DCC-interacting protein
UniProt Gene Name:APPL2  
UniProt Entry Name:DP13B_HUMAN
Anti-APPL2 Antibody (CAB14590)Western blot analysis of extracts of various cell lines, using APPL2 antibody (CAB14590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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