Cell Biology Antibodies 9
Anti-APOM Antibody (CAB5336)
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- SKU:
- CAB5336
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-APOM Antibody |
| Antibody SKU: | CAB5336 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-188 of human APOM (NP_061974.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, K-562, SW620, A-549, PC-3, U-251MG, Mouse liver, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-188 of human APOM (NP_061974.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CPEH SQLT TLGV DGKE FPEV HLGQ WYFI AGAA PTKE ELAT FDPV DNIV FNMA AGSA PMQL HLRA TIRM KDGL CVPR KWIY HLTE GSTD LRTE GRPD MKTE LFSS SCPG GIML NETG QGYQ RFLL YNRS PHPP EKCV EEFK SLTS CLDS KAFL LTPR NQEA CELS NN |
| Gene ID: | 55937 |
| Uniprot: | O95445 |
| Cellular Location: | Secreted |
| Calculated MW: | 13kDa/21kDa |
| Observed MW: | 25kDa |
| Synonyms: | APOM, G3a, HSPC336, NG20, apo-M |
| Background: | The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. |
| UniProt Protein Function: | APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 6p21.33 Cellular Component: integral to plasma membrane Molecular Function:antioxidant activity; lipid transporter activity; phospholipid binding Biological Process: cholesterol homeostasis; reverse cholesterol transport; response to glucose stimulus; cholesterol efflux; lipoprotein metabolic process |
| NCBI Summary: | The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012] |
| UniProt Code: | O95445 |
| NCBI GenInfo Identifier: | 17370872 |
| NCBI Gene ID: | 55937 |
| NCBI Accession: | O95445.2 |
| UniProt Secondary Accession: | O95445,Q5SRP4, Q9P046, Q9UMP6, B0UX98, |
| UniProt Related Accession: | O95445 |
| Molecular Weight: | 188 |
| NCBI Full Name: | Apolipoprotein M |
| NCBI Synonym Full Names: | apolipoprotein M |
| NCBI Official Symbol: | APOM |
| NCBI Official Synonym Symbols: | G3a; NG20; apo-M; HSPC336 |
| NCBI Protein Information: | apolipoprotein M; protein G3a; NG20-like protein; alternative name: G3a, NG20 |
| UniProt Protein Name: | Apolipoprotein M |
| UniProt Synonym Protein Names: | Protein G3a |
| Protein Family: | Apomucin |
| UniProt Gene Name: | APOM |
| UniProt Entry Name: | APOM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using APOM antibody (CAB5336) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded Human colon using APOM Rabbit pAb (CAB5336) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Mouse kidney using APOM Rabbit pAb (CAB5336) at dilution of 1:50 (40x lens). |
 | Immunofluorescence analysis of L929 cells using APOM Rabbit pAb (CAB5336) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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