Immunology Antibodies 2

Anti-APOBEC3F Antibody (CAB2507)

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SKU:
CAB2507
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-APOBEC3F Antibody
Antibody SKU:CAB2507
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human APOBEC3F (NP_660341.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, BxPC-3, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human APOBEC3F (NP_660341.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKPH FRNT VERM YRDT FSYN FYNR PILS RRNT VWLC YEVK TKGP SRPR LDAK IFRG QVYS QPEH H
Gene ID:200316
Uniprot:Q8IUX4
Cellular Location:Cytoplasm, P-body
Calculated MW:9kDa/11kDa/45kDa
Observed MW:45kDa
Synonyms:APOBEC3F, A3F, ARP8, BK150C2.4.MRNA, KA6
Background:This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:APOBEC3F: Host cellular restriction factor that may have antiviral activities against exogenous and endogenous viruses, as well as retrotransposons. After being packaged into HIV-1 virions, blocks productive infection by massively editing dC residues to dU on the DNA minus strand during reverse transcription. The editing of the minus strand DNA of HIV-1 during reverse transcription leads to G- to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Belongs to the cytidine and deoxycytidylate deaminase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA processing; Hydrolase; EC 3.5.4.-

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: apolipoprotein B mRNA editing enzyme complex; cytoplasm; ribonucleoprotein complex

Molecular Function:protein binding; zinc ion binding; RNA binding; cytidine deaminase activity

Biological Process: negative regulation of retroviral genome replication; base conversion or substitution editing; negative regulation of viral genome replication; innate immune response; cytidine deamination; positive regulation of defense response to virus by host; negative regulation of viral reproduction; defense response to virus

NCBI Summary:This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:Q8IUX4
NCBI GenInfo Identifier:161784334
NCBI Gene ID:200316
NCBI Accession:Q8IUX4.3
UniProt Secondary Accession:Q8IUX4,Q45F03, Q6ICH3, Q7Z2N2, Q7Z2N5, B0QYD4,
UniProt Related Accession:Q8IUX4
Molecular Weight:373
NCBI Full Name:DNA dC->
NCBI Synonym Full Names:apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F
NCBI Official Symbol:APOBEC3F  
NCBI Official Synonym Symbols:A3F; KA6; ARP8; BK150C2.4.MRNA  
NCBI Protein Information:DNA dC->dU-editing enzyme APOBEC-3F; induced upon T-cell activation; apolipoprotein B mRNA editing enzyme cytidine deaminase; apolipoprotein B editing enzyme catalytic polypeptide-like 3F; apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like
UniProt Protein Name:DNA dC->dU-editing enzyme APOBEC-3F
UniProt Synonym Protein Names:Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F; A3F
Protein Family:DNA dC->dU-editing enzyme
UniProt Gene Name:APOBEC3F  
UniProt Entry Name:ABC3F_HUMAN
Anti-APOBEC3F Antibody (CAB2507)Western blot analysis of extracts of various cell lines, using APOBEC3F antibody (CAB2507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-APOBEC3F Antibody (CAB2507)Immunohistochemistry of paraffin-embedded rat ovary using APOBEC3F antibody (CAB2507) at dilution of 1:100 (40x lens).
Anti-APOBEC3F Antibody (CAB2507)Immunohistochemistry of paraffin-embedded human lung cancer using APOBEC3F antibody (CAB2507) at dilution of 1:100 (40x lens).
Anti-APOBEC3F Antibody (CAB2507)Immunohistochemistry of paraffin-embedded mouse lung using APOBEC3F antibody (CAB2507) at dilution of 1:100 (40x lens).
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