Signal Transduction Antibodies 1

Anti-APH1A Antibody (CAB14666)

(No reviews yet) Write a Review
SKU:
CAB14666
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-APH1A Antibody
Antibody SKU:CAB14666
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human APH1A (NP_001071096.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:U-937, HT-29, HeLa
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human APH1A (NP_001071096.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TSGL TFLN PWYE ASLL PIYA VTVS MGLW AFIT AGGS LRSI QRSL LCRR QEDS RVMV YSAL RIPP ED
Gene ID:51107
Uniprot:Q96BI3
Cellular Location:Endoplasmic reticulum membrane, Golgi apparatus, Golgi stack membrane, Multi-pass membrane protein
Calculated MW:20kDa/26kDa/28kDa
Observed MW:29kDa
Synonyms:APH1A, 6530402N02Rik, APH-1, APH-1A, CGI-78
Background:This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants.
UniProt Protein Function:APH1A: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Belongs to the APH-1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; Membrane protein, integral; Apoptosis; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p36.13-q31.3

Cellular Component: endoplasmic reticulum; Golgi apparatus; integral to plasma membrane; membrane; plasma membrane

Molecular Function:protein binding

Biological Process: amyloid precursor protein catabolic process; ephrin receptor signaling pathway; membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; Notch receptor processing; Notch signaling pathway; positive regulation of apoptosis; positive regulation of catalytic activity; protein processing

NCBI Summary:This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants. [provided by RefSeq, Aug 2011]
UniProt Code:Q96BI3
NCBI GenInfo Identifier:37077707
NCBI Gene ID:51107
NCBI Accession:Q96BI3.1
UniProt Secondary Accession:Q96BI3,Q5TB22, Q5TB23, Q969R6, Q9BVG0, Q9Y386, B4DQK0
UniProt Related Accession:Q96BI3
Molecular Weight:20,977 Da
NCBI Full Name:Gamma-secretase subunit APH-1A
NCBI Synonym Full Names:aph-1 homolog A, gamma-secretase subunit
NCBI Official Symbol:APH1A  
NCBI Official Synonym Symbols:APH-1; APH-1A; CGI-78; 6530402N02Rik  
NCBI Protein Information:gamma-secretase subunit APH-1A
UniProt Protein Name:Gamma-secretase subunit APH-1A
UniProt Synonym Protein Names:Aph-1alpha; Presenilin-stabilization factor
Protein Family:Gamma-secretase
UniProt Gene Name:APH1A  
UniProt Entry Name:APH1A_HUMAN
Anti-APH1A Antibody (CAB14666)Western blot analysis of extracts of various cell lines, using APH1A antibody (CAB14666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-APH1A Antibody (CAB14666)Immunofluorescence analysis of L929 cells using APH1A Polyclonal Antibody (CAB14666) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products