Epigenetics & Nuclear Signaling Antibodies 1

APEX1/APE1 Rabbit Polyclonal Antibody (CAB1117)

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SKU:
CAB1117
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Synonyms:
APE, APX, APE1, APEN, APEX, HAP1, REF1, APEX1/APE1
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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APEX1/APE1 Antibody (CAB1117)

The APEX1/APE1 Antibody (CAB1117) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By binding to the APEX1 protein, this antibody enables the detection and analysis of APEX1 in various cellular contexts, making it an essential reagent for studies in DNA repair mechanisms, oxidative stress response, and cancer biology.APEX1, also known as APE1 or Ref-1, is a key player in maintaining genomic stability and regulating gene expression through its dual functions in DNA repair and transcriptional regulation.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:APEX1/APE1 Antibody
SKU:CAB1117
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MPKR GKKG AVAE DGDE LRTE PEAK KSKT AAKK NDKE AAGE GPAL YEDP PDQK TSPS GKPA TLKI CSWN VDGL RAWI KKKG LDWV KEEA PDIL CLQE TKCS ENKL PAEL QELP GLSH QYWS APSD KEGY SGVG LLSR QCPL KVSY GIGD EEHD QEGR VIVA EFDS FVLV TAYV PNAG RGLV RLEY RQRW DEAF RKFL KGLA SRKP LVLC GDLN VAHE EIDL RNPK GNKK NAGF TPQE RQGF GELL QAVP LADS FRHL YPNT PYAY TFWT YMMN ARSK NVGW RLDY FLLS HSLL PALC DSKI RSKA LGSD HCPI TLYL AL
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:APE, APX, APE1, APEN, APEX, HAP1, REF1, APEX1/APE1
Positive Sample:HeLa, HepG2
Cellular Localization:Cytoplasm, Endoplasmic Reticulum, Mitochondrion, Nucleus, Nucleus Speckle, Nucleolus.
Calculated MW:36kDa
Observed MW:39kDa

The APEX gene encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. The AP sites are the most frequent pre-mutagenic lesions that can prevent normal DNA replication. Splice variants have been found for this gene; all encode the same protein. Disruptions in the biological functions related to APEX are associated with many various malignancies and neurodegenerative diseases.

Purification MethodAffinity purification
Gene ID328
RRIDAB_2758444
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using APEX1/APE1 Rabbit pAb (CAB1117) . Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product ImageImmunofluorescence analysis of HeLa cells using APEX1/APE1 Rabbit pAb (CAB1117). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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