Name: Anti-AP3B1 Antibody (CAB7019)
Brand: Cell Biology Antibodies 10
SKU: CAB7019
Availability: OutOfStock

Antibody Name:	Anti-AP3B1 Antibody
Antibody SKU:	CAB7019
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 895-1094 of human AP3B1 (NP_003655.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	SKOV3, SW620, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 895-1094 of human AP3B1 (NP_003655.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FGDK MVSI QITL NNTT DRKI ENIH IGEK KLPI GMKM HVFN PIDS LEPE GSIT VSMG IDFC DSTQ TASF QLCT KDDC FNVN IQPP VGEL LLPV AMSE KDFK KEQG VLTG MNET SAVI IAAP QNFT PSVI FQKV VNVA NVGA VPSG QDNI HRFA AKTV HSGS LMLV TVEL KEGS TAQL IINT EKTV IGSV LLRE LKPV LSQG
Gene ID:	8546
Uniprot:	O00203
Cellular Location:	Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus, Peripheral membrane protein, clathrin-coated vesicle membrane
Calculated MW:	116kDa/121kDa
Observed MW:	132kDa

Synonyms:

AP3B1, ADTB3, ADTB3A, HPS, HPS2, PE

Background:

This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is part of the heterotetrameric AP-3 protein complex which interacts with the scaffolding protein clathrin. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 2. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	AP3B1: a subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. Mutations in AP3B1 cause defects of various cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes, and are the cause of Hermansky-Pudlak syndrome 2. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Vesicle Chromosomal Location of Human Ortholog: 5q14.1 Cellular Component: Golgi apparatus; membrane; lysosomal membrane; clathrin coated vesicle membrane Molecular Function:GTP-dependent protein binding; protein phosphatase binding Biological Process: intracellular protein transport; protein targeting to lysosome; melanosome organization and biogenesis; anterograde synaptic vesicle transport; antigen processing and presentation, exogenous lipid antigen via MHC class Ib; positive regulation of NK T cell differentiation; blood coagulation; anterograde axon cargo transport Disease: Hermansky-pudlak Syndrome 2
NCBI Summary:	This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is part of the heterotetrameric AP-3 protein complex which interacts with the scaffolding protein clathrin. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012]
UniProt Code:	O00203
NCBI GenInfo Identifier:	254763431
NCBI Gene ID:	8546
NCBI Accession:	O00203.3
UniProt Secondary Accession:	O00203,O00580, Q7Z393, Q9HD66, E5RJ68,
UniProt Related Accession:	O00203
Molecular Weight:	116,195 Da
NCBI Full Name:	AP-3 complex subunit beta-1
NCBI Synonym Full Names:	adaptor-related protein complex 3, beta 1 subunit
NCBI Official Symbol:	AP3B1
NCBI Official Synonym Symbols:	PE; HPS; HPS2; ADTB3; ADTB3A
NCBI Protein Information:	AP-3 complex subunit beta-1; beta-3A-adaptin; AP-3 complex beta-3A subunit; adaptor protein complex AP-3 subunit beta-1; adaptor-related protein complex 3 subunit beta-1; clathrin assembly protein complex 3 beta-1 large chain
UniProt Protein Name:	AP-3 complex subunit beta-1
UniProt Synonym Protein Names:	Adaptor protein complex AP-3 subunit beta-1; Adaptor-related protein complex 3 subunit beta-1; Beta-3A-adaptin; Clathrin assembly protein complex 3 beta-1 large chain
Protein Family:	AP-3 complex
UniProt Gene Name:	AP3B1
UniProt Entry Name:	AP3B1_HUMAN

Western blot analysis of extracts of various cell lines, using AP3B1 antibody (CAB7019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

AP3B1 Antibody (PACO07768)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)