Cell Biology Antibodies 2

Anti-AP2S1 Antibody (CAB12270)

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SKU:
CAB12270
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AP2S1 Antibody
Antibody SKU:CAB12270
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human AP2S1 (NP_004060.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, LO2, SH-SY5Y, Mouse brain, Mouse kidney, Mouse spleen, Rat brain, Rat spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human AP2S1 (NP_004060.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MIRF ILIQ NRAG KTRL AKWY MQFD DDEK QKLI EEVH AVVT VRDA KHTN FVEF RNFK IIYR RYAG LYFC ICVD VNDN NLAY LEAI HNFV EVLN EYFH NVCE LDLV FNFY KVYT VVDE MFLA GEIR ETSQ TKVL KQLL MLQS LE
Gene ID:1175
Uniprot:P53680
Cellular Location:Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit
Calculated MW:12kDa/17kDa
Observed MW:20kDa
Synonyms:AP2S1, AP17, CLAPS2, FBH3, FBHOk, HHC3
Background:One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:AP2S1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes small subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function:transporter activity; protein transporter activity

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; synaptic transmission; clathrin cage assembly; axon guidance; intracellular protein transport; nerve growth factor receptor signaling pathway; viral reproduction; regulation of endocytosis; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus

Disease: Hypocalciuric Hypercalcemia, Familial, Type Iii

NCBI Summary:One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Code:P53680
NCBI GenInfo Identifier:51338780
NCBI Gene ID:1175
NCBI Accession:P53680.2
UniProt Secondary Accession:P53680,O75977, Q6PK67, B2R4Z4,
UniProt Related Accession:P53680
Molecular Weight:12,417 Da
NCBI Full Name:AP-2 complex subunit sigma
NCBI Synonym Full Names:adaptor-related protein complex 2, sigma 1 subunit
NCBI Official Symbol:AP2S1  
NCBI Official Synonym Symbols:AP17; FBH3; HHC3; FBHOk; CLAPS2  
NCBI Protein Information:AP-2 complex subunit sigma; sigma2-adaptin; HA2 17 kDa subunit; clathrin coat assembly protein AP17; clathrin coat-associated protein AP17; adaptor protein complex AP-2 subunit sigma; plasma membrane adaptor AP-2 17 kDa protein; clathrin assembly protein 2 sigma small chain; adaptor-related protein complex 2 subunit sigma; clathrin-associated/assembly/adaptor protein, small 2 (17kD)
UniProt Protein Name:AP-2 complex subunit sigma
UniProt Synonym Protein Names:Adaptor protein complex AP-2 subunit sigma; Adaptor-related protein complex 2 subunit sigma; Clathrin assembly protein 2 sigma small chain; Clathrin coat assembly protein AP17; Clathrin coat-associated protein AP17; HA2 17 kDa subunit; Plasma membrane adaptor AP-2 17 kDa protein; Sigma2-adaptin
Protein Family:AP-2 complex
UniProt Gene Name:AP2S1  
UniProt Entry Name:AP2S1_HUMAN
Anti-AP2S1 Antibody (CAB12270)Western blot analysis of extracts of various cell lines, using AP2S1 antibody (CAB12270) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
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