Cell Biology Antibodies 2

Anti-AP1S1 Antibody (CAB10558)

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SKU:
CAB10558
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AP1S1 Antibody
Antibody SKU:CAB10558
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human AP1S1 (NP_001274.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:SW480
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human AP1S1 (NP_001274.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMRF MLLF SRQG KLRL QKWY LATS DKER KKMV RELM QVVL ARKP KMCS FLEW RDLK VVYK RYAS LYFC CAIE GQDN ELIT LELI HRYV ELLD KYFG SVCE LDII FNFE KAYF ILDE FLMG
Gene ID:1174
Uniprot:P61966
Cellular Location:Cytoplasmic side, Cytoplasmic vesicle membrane, Golgi apparatus, Membrane, Peripheral membrane protein, clathrin-coated pit
Calculated MW:16kDa/18kDa
Observed MW:19kDa
Synonyms:AP1S1, AP19, CLAPS1, EKV3, MEDNIK, SIGMA1A
Background:The protein encoded by this gene is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle.
UniProt Protein Function:AP1S1: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Belongs to the adaptor complexes small subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: AP-1 adaptor complex; cytoplasmic vesicle membrane; cytosol; Golgi apparatus; Golgi membrane; intracellular membrane-bound organelle; lysosomal membrane; membrane; trans-Golgi network membrane

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; receptor-mediated endocytosis; regulation of defense response to virus by virus; response to virus

Disease: Mental Retardation, Enteropathy, Deafness, Peripheral Neuropathy, Ichthyosis, And Keratoderma

NCBI Summary:The protein encoded by this gene is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle. [provided by RefSeq, Jul 2008]
UniProt Code:P61966
NCBI GenInfo Identifier:48428719
NCBI Gene ID:1174
NCBI Accession:P61966.1
UniProt Secondary Accession:P61966,P82267, Q00382, Q53YA7, Q9BTN4, Q9UDW9, B2R5D8
UniProt Related Accession:P61966
Molecular Weight:19kDa
NCBI Full Name:AP-1 complex subunit sigma-1A
NCBI Synonym Full Names:adaptor related protein complex 1 sigma 1 subunit
NCBI Official Symbol:AP1S1  
NCBI Official Synonym Symbols:AP19; EKV3; CLAPS1; MEDNIK; SIGMA1A  
NCBI Protein Information:AP-1 complex subunit sigma-1A
UniProt Protein Name:AP-1 complex subunit sigma-1A
UniProt Synonym Protein Names:Adaptor protein complex AP-1 subunit sigma-1A; Adaptor-related protein complex 1 subunit sigma-1A; Clathrin assembly protein complex 1 sigma-1A small chain; Clathrin coat assembly protein AP19; Golgi adaptor HA1/AP1 adaptin sigma-1A subunit; HA1 19 kDa subunit; Sigma 1a subunit of AP-1 clathrin; Sigma-adaptin 1A; Sigma1A-adaptin
Protein Family:AP-1 complex
UniProt Gene Name:AP1S1  
Anti-AP1S1 Antibody (CAB10558)Western blot analysis of extracts of SW480 cells, using AP1S1 antibody (CAB10558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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