KO Validated Antibodies 1

Anti-ANXA2 Antibody (CAB12397)[KO Validated]

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SKU:
CAB12397
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-ANXA2 Antibody [KO Validated]
Antibody SKU:CAB12397
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human ANXA2 (NP_004030.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, HepG2, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human ANXA2 (NP_004030.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSTV HEIL CKLS LEGD HSTP PSAY GSVK AYTN FDAE RDAL NIET AIKT KGVD EVTI VNIL TNRS NAQR QDIA FAYQ RRTK KELA SALK SALS GHLE TVIL GLLK TPAQ YDAS ELKA SMKG LGTD EDSL IEII CSRT NQEL QEIN RVYK EMYK TDLE KDII SDTS GDFR KLMV ALAK GRRA EDGS VIDY ELID QDAR DLYD AGVK RKGT DVPK WISI MTER SVPH LQKV FDRY KSYS PYDM LESI RKEV KGDL ENAF LNLV QCIQ NKPL YFAD RLYD SMKG KGTR DKVL IRIM VSRS EVDM LKIR SEFK RKYG KSLY YYIQ QDTK GDYQ KALL YLCG GDD
Gene ID:302
Uniprot:P07355
Cellular Location:Melanosome, Secreted, basement membrane, extracellular matrix, extracellular space
Calculated MW:38kDa/40kDa
Observed MW:38kDa
Synonyms:ANXA2, ANX2, ANX2L4, CAL1H, HEL-S-270, LIP2, LPC2, LPC2D, P36, PAP-IV
Background:This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:ANXA2: a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. Heterotetramer containing 2 light chains of S100A10 2 heavy chains of ANXA2. May cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: extrinsic to plasma membrane; extracellular space; cell surface; protein complex; lysosomal membrane; late endosome membrane; early endosome; lipid particle; cell cortex; lipid raft; ruffle; extracellular matrix; membrane; perinuclear region of cytoplasm; plasma membrane; melanosome; basement membrane; midbody; nucleus; sarcolemma; vesicle; endosome

Molecular Function:protein binding; phosphatidylinositol-4,5-bisphosphate binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; cytoskeletal protein binding; calcium ion binding; calcium-dependent protein binding; Rab GTPase binding

Biological Process: fibrinolysis; positive regulation of fibroblast proliferation; collagen fibril organization; negative regulation of catalytic activity; positive regulation of vesicle fusion; protein heterotetramerization; positive regulation of binding; positive regulation of protein amino acid phosphorylation; angiogenesis; lipid raft formation; body fluid secretion; membrane budding

NCBI Summary:This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P07355
NCBI GenInfo Identifier:113950
NCBI Gene ID:302
NCBI Accession:P07355.2
UniProt Secondary Accession:P07355,Q567R4, Q6N0B3, Q8TBV2, Q96DD5, Q9UDH8,
UniProt Related Accession:P07355
Molecular Weight:40,411 Da
NCBI Full Name:Annexin A2
NCBI Synonym Full Names:annexin A2
NCBI Official Symbol:ANXA2  
NCBI Official Synonym Symbols:P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270  
NCBI Protein Information:annexin A2; annexin-2; protein I; annexin II; lipocortin II; chromobindin 8; chromobindin-8; calpactin I heavy chain; calpactin-1 heavy chain; calpactin I heavy polypeptide; placental anticoagulant protein IV; epididymis secretory protein Li 270
UniProt Protein Name:Annexin A2
UniProt Synonym Protein Names:Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; Chromobindin-8; Lipocortin II; Placental anticoagulant protein IV; PAP-IV; Protein I; p36
Protein Family:Annexin
UniProt Gene Name:ANXA2  
UniProt Entry Name:ANXA2_HUMAN
Anti-ANXA2 Antibody [KO Validated] (CAB12397)Western blot analysis of extracts of various cell lines, using ANXA2 Antibody (CAB12397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-ANXA2 Antibody [KO Validated] (CAB12397)Western blot analysis of extracts from normal (control) and ANXA2 knockout (KO) HeLa cells, using ANXA2 antibody (CAB12397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-ANXA2 Antibody [KO Validated] (CAB12397)Immunohistochemistry of paraffin-embedded rat heart using ANXA2 antibody (CAB12397) at dilution of 1:100 (40x lens).
Anti-ANXA2 Antibody [KO Validated] (CAB12397)Immunohistochemistry of paraffin-embedded mouse liver using ANXA2 antibody (CAB12397) at dilution of 1:100 (40x lens).
Anti-ANXA2 Antibody [KO Validated] (CAB12397)Immunohistochemistry of paraffin-embedded mouse heart using ANXA2 antibody (CAB12397) at dilution of 1:100 (40x lens).
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