Signal Transduction Antibodies 2

Anti-ANO2 Antibody (CAB3684)

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SKU:
CAB3684
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-ANO2 Antibody
Antibody SKU:CAB3684
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ANO2 (NP_001265526.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ANO2 (NP_001265526.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPED IPLL PGSP RRLS PQAG SRGG QGPK HGQQ CLKM PGPR APGL QGGS NRDP GQPC GGES TRSS SVIN NYLD ANEP VSLE ARLS RMHF HDSQ RKVD YVLA YHYR KRGV HLAQ GFPG HSLA IVSN GETG KEPH AGGP GDIE LGPL DALE EERK EQRE EFEH NLME AGLE LEKD LENK SQGS IFVR IHAP WQVL AREA EFLK
Gene ID:57101
Uniprot:Q9NQ90
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:113kDa
Observed MW:114kDa
Synonyms:ANO2, C12orf3, TMEM16B
Background:
UniProt Protein Function:ANO2: Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transporter, ion channel; Transporter; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: nucleoplasm; nonmotile primary cilium; plasma membrane

Molecular Function:protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity

Biological Process: transmembrane transport

NCBI Summary:ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).[supplied by OMIM, Jan 2011]
UniProt Code:Q9NQ90
NCBI GenInfo Identifier:262527528
NCBI Gene ID:57101
NCBI Accession:Q9NQ90.2
UniProt Secondary Accession:Q9NQ90,Q9H847, C4N787,
UniProt Related Accession:Q9NQ90
Molecular Weight:1003
NCBI Full Name:Anoctamin-2
NCBI Synonym Full Names:anoctamin 2, calcium activated chloride channel
NCBI Official Symbol:ANO2  
NCBI Official Synonym Symbols:C12orf3; TMEM16B  
NCBI Protein Information:anoctamin-2; transmembrane protein 16B (eight membrane-spanning domains)
UniProt Protein Name:Anoctamin-2
UniProt Synonym Protein Names:Transmembrane protein 16B
Protein Family:Anoctamin
UniProt Gene Name:ANO2  
UniProt Entry Name:ANO2_HUMAN
Anti-ANO2 Antibody (CAB3684)Western blot analysis of extracts of mouse brain, using ANO2 antibody (CAB3684) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-ANO2 Antibody (CAB3684)Immunofluorescence analysis of Y79 cells using ANO2 Polyclonal Antibody (CAB3684) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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