Signal Transduction Antibodies 1

Anti-ANO1 Antibody (CAB10498)

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SKU:
CAB10498
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-ANO1 Antibody
Antibody SKU:CAB10498
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 628-731 of human ANO1 (NP_060513.5).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HCT116, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 628-731 of human ANO1 (NP_060513.5).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FKGR FVGR PGDY VYIF RSFR MEEC APGG CLME LCIQ LSII MLGK QLIQ NNLF EIGI PKMK KLIR YLKL KQQS PPDH EECV KRKQ RYEV DYNL EPFA GLTP EYME
Gene ID:55107
Uniprot:Q5XXA6
Cellular Location:Cell membrane, Cytoplasm, Multi-pass membrane protein
Calculated MW:74kDa/97kDa/114kDa
Observed MW:150kDa
Synonyms:ANO1, DOG1, ORAOV2, TAOS2, TMEM16A
Background:
UniProt Protein Function:ANO1: Acts as a calcium-activated chloride channel. Required for normal tracheal development. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Transporter, ion channel; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: apical plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function:protein binding; protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity; calcium activated cation channel activity

Biological Process: regulation of membrane potential; multicellular organismal development; chloride transport; transmembrane transport; cation transport

UniProt Code:Q5XXA6
NCBI GenInfo Identifier:74708278
NCBI Gene ID:55107
NCBI Accession:Q5XXA6.1
UniProt Secondary Accession:Q5XXA6,Q8IYY8, Q8N7V3, A8KAM3,
UniProt Related Accession:Q5XXA6
Molecular Weight:
NCBI Full Name:Anoctamin-1
NCBI Synonym Full Names:anoctamin 1, calcium activated chloride channel
NCBI Official Symbol:ANO1  
NCBI Official Synonym Symbols:DOG1; TAOS2; ORAOV2; TMEM16A  
NCBI Protein Information:anoctamin-1; Ca2+-activated Cl- channel; oral cancer overexpressed 2; tumor-amplified and overexpressed sequence 2; discovered on gastrointestinal stromal tumors protein 1; transmembrane protein 16A (eight membrane-spanning domains)
UniProt Protein Name:Anoctamin-1
UniProt Synonym Protein Names:Discovered on gastrointestinal stromal tumors protein 1; Oral cancer overexpressed protein 2; Transmembrane protein 16A; Tumor-amplified and overexpressed sequence 2
Protein Family:Anoctamin
UniProt Gene Name:ANO1  
UniProt Entry Name:ANO1_HUMAN
Anti-ANO1 Antibody (CAB10498)Western blot analysis of extracts of various cell lines, using ANO1 antibody (CAB10498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-ANO1 Antibody (CAB10498)Immunofluorescence analysis of U2OS cells using ANO1 antibody (CAB10498) at dilution of 1:100. Blue: DAPI for nuclear staining.
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