Name: Anti-ANKLE2 Antibody (CAB17654)
Brand: Cell Biology Antibodies 13
SKU: CAB17654
Availability: OutOfStock

Antibody Name:	Anti-ANKLE2 Antibody
Antibody SKU:	CAB17654
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human ANKLE2 (NP_055929.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Jurkat, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human ANKLE2 (NP_055929.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CRYN VMHV AAKE NQAS ICQL TLDV LENP DFMR LMYP DDDE AMLQ KRIR YVVD LYLN TPDK MGYD TPLH FACK FGNA DVVN VLSS HHLI VKNS RNKY DKTP E
Gene ID:	23141
Uniprot:	Q86XL3
Cellular Location:
Calculated MW:
Observed MW:	104kDa

Western blot - ANKLE2 Rabbit pAb (CAB17654)

Synonyms:	KIAA0692, LEMD7, Lem4, MCPH16, ANKLE2
Background:	This gene encodes a member of the LEM family of inner nuclear membrane proteins. The encoded protein functions as a mitotic regulator through postmitotic formation of the nuclear envelope. Mutations in this gene cause morphology defects in the nuclear envelope and BAF hyperphosphorylation. [provided by RefSeq, Mar 2014]

UniProt Protein Function:	ANKLE2: Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex. Belongs to the ANKLE2 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 12q24.33 Cellular Component: endoplasmic reticulum membrane; membrane; integral to endoplasmic reticulum membrane Molecular Function:protein binding; protein phosphatase 2A binding; protein phosphatase type 2A regulator activity Biological Process: mitosis; mitotic nuclear envelope reassembly; cell division; regulation of catalytic activity; negative regulation of phosphorylation; mitotic cell cycle; positive regulation of protein amino acid dephosphorylation
NCBI Summary:	This gene encodes a member of the LEM family of inner nuclear membrane proteins. The encoded protein functions as a mitotic regulator through postmitotic formation of the nuclear envelope. Mutations in this gene cause morphology defects in the nuclear envelope and BAF hyperphosphorylation. [provided by RefSeq, Mar 2014]
UniProt Code:	Q86XL3
NCBI GenInfo Identifier:	148664230
NCBI Gene ID:	23141
NCBI Accession:	NP_055929.1
UniProt Secondary Accession:	Q86XL3,O75176, Q6P6A5, Q8TAZ9, Q96DH4, A8KAG3, B3KN97 B3KSF8,
UniProt Related Accession:	Q86XL3
Molecular Weight:	31,982 Da
NCBI Full Name:	ankyrin repeat and LEM domain-containing protein 2
NCBI Synonym Full Names:	ankyrin repeat and LEM domain containing 2
NCBI Official Symbol:	ANKLE2
NCBI Official Synonym Symbols:	Lem4; LEMD7; KIAA0692
NCBI Protein Information:	ankyrin repeat and LEM domain-containing protein 2
UniProt Protein Name:	Ankyrin repeat and LEM domain-containing protein 2
UniProt Synonym Protein Names:	LEM domain-containing protein 4
Protein Family:	Ankyrin repeat and LEM domain-containing protein
UniProt Gene Name:	ANKLE2
UniProt Entry Name:	ANKL2_HUMAN