Cell Biology Antibodies 11
Anti-Angiomotin Antibody (CAB8075)
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- SKU:
- CAB8075
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | [KO Validated] AMOT Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8075 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human AMOT (NP_573572.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, A-431, M21, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human AMOT (NP_573572.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPRA QPSS ASYQ PVPA DPFA IVSR AQQM VEIL SDEN RNLR QELE GCYE KVAR LQKV ETEI QRVS EAYE NLVK SSSK REAL EKAM RNKL EGEI RRMH DFNR DLRE RLET ANKQ LAEK EYEG SEDT RKTI SQLF AKNK ESQR EKEK LEAE LATA RSTN EDQR RHIE IRDQ ALSN AQAK VVKL EEEL KKKQ VYVD KVEK MQQA LVQL QAAC EKRE QLEH RLRT RLER ELES LRIQ QRQG NCQP TNVS EYNA AALM ELLR EKEE RILA LEAD MTKW EQKY LEEN VMRH FALD AAAT VAAQ RDTT |
| Gene ID: | 154796 |
| Uniprot: | Q4VCS5 |
| Cellular Location: | Cell junction, tight junction |
| Calculated MW: | 72kDa/118kDa |
| Observed MW: | 70kDa |
| Synonyms: | AMOT |
| Background: | This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | AMOT: Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Belongs to the angiomotin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: Xq23 Cellular Component: actin filament; cytosol; endocytic vesicle; external side of plasma membrane; lamellipodium; ruffle; signalosome Molecular Function:angiostatin binding; protein binding; receptor activity Biological Process: actin cytoskeleton organization and biogenesis; negative regulation of angiogenesis; regulation of cell migration |
| NCBI Summary: | This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q4VCS5 |
| NCBI GenInfo Identifier: | 74753814 |
| NCBI Gene ID: | 154796 |
| NCBI Accession: | Q4VCS5.1 |
| UniProt Secondary Accession: | Q4VCS5,Q504X5, Q9HD27, Q9UPT1, |
| UniProt Related Accession: | Q4VCS5 |
| Molecular Weight: | 72,540 Da |
| NCBI Full Name: | Angiomotin |
| NCBI Synonym Full Names: | angiomotin |
| NCBI Official Symbol: | AMOT |
| NCBI Protein Information: | angiomotin |
| UniProt Protein Name: | Angiomotin |
| Protein Family: | Angiomotin |
| UniProt Gene Name: | AMOT |
| UniProt Entry Name: | AMOT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AMOT antibody (CAB8075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts from normal (control) and AMOT Rabbit pAb knockout (KO) 293T cells, using AMOT Rabbit pAb antibody (CAB8075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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