Anti-Amyloid Beta 1-42 Oligomer Antibody [2F9]

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SKU:
STMA00224
Antibody Type:
Recombinant Monoclonal
Applications:
WB
Applications:
ELISA
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
  • Western blot analysis comparing detection of Amyloid Beta 1-42 constructs (amyloid beta 1-42 peptide; amyloid beta 1-42 oligomers; amyloid beta 1-42 pre-formed fibrils; amyloid beta pyroglutamate 3-42 pre-formed fibrils). Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (STMA00224, clone 2F9) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10), at 1:10,000 & 1:1000.
  • Sandwich ELISA analysis comparing detection of Amyloid Beta constructs. The range tested for each construct was 0 to 5000 pg/mL, and the standard curve was prepared using amyloid beta 1-42 oligomers. Within this range, no cross-reactivity with monomeric peptide & APP 751 was observed.
  • Dot blot analysis comparing detection of amyloid beta 1-42 constructs, alpha synuclein wildtype constructs, and tau constructs. Of note is that STMA00224 does not bind APP 751 or other protein constructs such as alpha synuclein and tau. Block: 5% skim milk.
  • Dot blot analysis comparing detection of amyloid beta constructs. Concentrations tested on each blot are 20 & 2ng, respectively. Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (STMA00224, clone 2F9) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10) at 1:10,000 & 1:1000, respectively, for 1/2 hour at RT.
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Description

Amyloid Beta 1-42 Oligomer Antibody [2F9]

Amyloid Beta 1-42 Oligomer Antibody [2F9] is a rabbit recombinant monoclonal antibody directed against Amyloid Beta 1-42 Oligomer, offered by Assay Genie for research applications. Reported applications include WB and ELISA. Reported reactivity: Human. Supplied as IgG, purified by Protein A.

Amyloid beta (Aβ) peptide, particularly the 1-42 isoform (Aβ1-42), plays a central role in the pathogenesis of Alzheimer’s disease (AD). Aβ is generated through proteolytic cleavage of the amyloid precursor protein (APP) and can self-assemble into a range of aggregated species, including oligomers, protofibrils, fibrils, and ultimately extracellular plaques. Among these, Aβ1-42 oligomers are increasingly recognized as the most neurotoxic species, contributing to synaptic dysfunction, neuroinflammation, and cognitive decline. This amyloid beta oligomer antibody has been validated for high specificity toward amyloid beta aggregates, distinguishing them from aggregated Tau and Alpha Synuclein proteins. This specificity is critical for accurately detecting early-stage, soluble aggregates that precede plaque formation and are believed to drive early neurodegenerative changes in AD. Tools that selectively recognize aggregated amyloid beta are essential for advancing both basic research and translational efforts in Alzheimer’s disease. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg, 12 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:Amyloid Beta 1-42 Oligomer Antibody [2F9]
Product SKU:STMA00224
Size:100 µg, 12 µg
Target:Amyloid Beta 1-42 Oligomer
Host Species:Rabbit
Antibody Type:Recombinant Monoclonal
Clone ID:2F9
Research Areas:Neuroscience, Neurodegeneration, Alzheimer's Disease, Amyloid
Buffer:PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, ELISA
Antibody Isotype:IgG
Purification:Protein A Purified
Concentration:1mg/ml
Reactivity:Human
Recommended Dilution:WB, ELISA (1:1000 - 1:10,000); optimal dilutions for assays should be determined by the user.
Specificity:Amyloid Beta Aggregates
Guarantee:12 months from date of dispatch
Immunogen:Amyloid beta 1-42 oligomers
Immunogen Species:Human
Gene ID:351 (APP)
Swiss-Prot:P05067
Cellular Localization:Membrane (Secreted)
Tissue Specificity:CNS, Blood
Alternative Names:Amyloid Beta 1-42 Oligomer, Aβ1-42 Oligomer, Abeta Oligomers, Amyloid beta peptide 1-42, Aβ42, Beta-amyloid protein 42, Beta-APP42, Amyloid beta A4 protein, APP, ABPP, APPI, Non-A4 component of amyloid precursor, Alzheimer disease amyloid protein
References:1. Panza et al. 2019. Nat Rev Neurol. 15:73-88 https://doi.org/10.1038/s41582-018-0116-6 2. Stine et al. 2003. JBC. 278(13):11612-22. doi: 10.1074/jbc.M210207200 3. Ahmed et al. 2010. Nature Structural & Molecular Biology. 17(5):561 doi: 10.1038/nsmb.1799
Note:For research use only.
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