Cell Biology Antibodies 2

Anti-AMACR Antibody (CAB1130)

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SKU:
CAB1130
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AMACR Antibody
Antibody SKU:CAB1130
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human AMACR (NP_976316.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LNCap, Mouse kindey, Mouse liver, Rat kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human AMACR (NP_976316.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MALQ GISV VELS GLAP GPFC AMVL ADFG ARVV RVDR PGSR YDVS RLGR GKRS LVLD LKQP RGAA VLRR LCKR SDVL LEPF RRGV MEKL QLGP EILQ RENP RLIY ARLS GFGQ SGSF CRLA GHDI NYLA LSGG RNSI FKFF SVEN SEIE SVGS TSRT EHVG WWST FLYD LQDS RWGI HGCW SNRT PVLR AADQ RTWT KV
Gene ID:23600
Uniprot:Q9UHK6
Cellular Location:Mitochondrion, Peroxisome
Calculated MW:22kDa/25kDa/42kDa/43kDa
Observed MW:42kDa/43kDa
Synonyms:AMACR, AMACRD, CBAS4, P504S, RACE, RM
Background:This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene.
UniProt Protein Function:AMACR: Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. Belongs to the CaiB/BaiF CoA-transferase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Lipid Metabolism - primary bile acid biosynthesis; EC 5.1.99.4; Isomerase; Mitochondrial

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: peroxisomal matrix; mitochondrion; cytoplasm; peroxisome

Molecular Function:receptor binding; alpha-methylacyl-CoA racemase activity

Biological Process: bile acid biosynthetic process; fatty acid beta-oxidation using acyl-CoA oxidase; bile acid metabolic process; cellular lipid metabolic process

Disease: Bile Acid Synthesis Defect, Congenital, 4; Alpha-methylacyl-coa Racemase Deficiency

NCBI Summary:This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]
UniProt Code:Q9UHK6
NCBI GenInfo Identifier:313104070
NCBI Gene ID:23600
NCBI Accession:Q9UHK6.2
UniProt Secondary Accession:Q9UHK6,O43673, Q3KT79, Q96GH1, Q9Y3Q1, A5YM47, B8Y916 B8Y918, F8W9N1,
UniProt Related Accession:Q9UHK6
Molecular Weight:382
NCBI Full Name:Alpha-methylacyl-CoA racemase
NCBI Synonym Full Names:alpha-methylacyl-CoA racemase
NCBI Official Symbol:AMACR  
NCBI Official Synonym Symbols:RM; RACE; CBAS4; AMACRD  
NCBI Protein Information:alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase
UniProt Protein Name:Alpha-methylacyl-CoA racemase
UniProt Synonym Protein Names:2-methylacyl-CoA racemase
Protein Family:Alpha-methylacyl-CoA racemase
UniProt Gene Name:AMACR  
UniProt Entry Name:AMACR_HUMAN
Anti-AMACR Antibody (CAB1130)Western blot analysis of extracts of various cell lines, using AMACR antibody (CAB1130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-AMACR Antibody (CAB1130)Immunohistochemistry of paraffin-embedded human prostate cancer using AMACR Rabbit pAb (CAB1130) at dilution of 1:100 (40x lens).
Anti-AMACR Antibody (CAB1130)Immunofluorescence analysis of rat kidney using AMACR Rabbit pAb (CAB1130) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-AMACR Antibody (CAB1130)Immunofluorescence analysis of mouse kidney using AMACR Rabbit pAb (CAB1130) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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