Name: Anti-ALYREF Antibody (CAB6010)
Brand: Immunology Antibodies 2
SKU: CAB6010
Availability: OutOfStock

Antibody Name:	Anti-ALYREF Antibody
Antibody SKU:	CAB6010
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human ALYREF

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A-549, B cells, SKOV3, HL-60, Mouse testis, Mouse spleen, Mouse thymus, Rat brain

Immunogen:	Recombinant protein of human ALYREF
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	10189
Uniprot:	Q86V81
Cellular Location:	Cytoplasm, Nucleus, Nucleus speckle
Calculated MW:	26kDa
Observed MW:	30kDa

Synonyms:	ALYREF, ALY, ALY/REF, BEF, REF, THOC4
Background:	The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.

UniProt Protein Function:	Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability.
NCBI Summary:	The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]
UniProt Code:	Q86V81
NCBI GenInfo Identifier:	48429165
NCBI Gene ID:	10189
NCBI Accession:	Q86V81.3
UniProt Secondary Accession:	Q86V81,O43672,
UniProt Related Accession:	Q86V81
Molecular Weight:	26 kDa
NCBI Full Name:	THO complex subunit 4
NCBI Synonym Full Names:	Aly/REF export factor
NCBI Official Symbol:	ALYREF
NCBI Official Synonym Symbols:	ALY; BEF; REF; THOC4; ALY/REF
NCBI Protein Information:	THO complex subunit 4
UniProt Protein Name:	THO complex subunit 4
UniProt Synonym Protein Names:	Ally of AML-1 and LEF-1; Aly/REF export factor; Transcriptional coactivator Aly/REF; bZIP-enhancing factor BEF
Protein Family:	Alytesin
UniProt Gene Name:	ALYREF

	Western blot analysis of extracts of various cell lines, using ALYREF antibody (CAB6010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded Rat lung using ALYREF Rabbit pAb (CAB6010) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse liver using ALYREF Rabbit pAb (CAB6010) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using ALYREF Polyclonal Antibody (CAB6010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using ALYREF Polyclonal Antibody (CAB6010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.