Signal Transduction Antibodies 1

Alkaline Phosphatase (ALPL) Rabbit Polyclonal Antibody (CAB1080)

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SKU:
CAB1080
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
Reactivity:
Human, Mouse, Rat
Synonyms:
HOPS, HPPA, HPPC, HPPI, HPPO, TNAP, TNALP, APTNAP, TNSALP, AP-TNAP, TNS-ALP, Alkaline Phosphatase (ALPL)
Research Use:
Signal Transduction
Frequently bought together:

Description

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Alkaline Phosphatase (ALPL) Antibody (CAB1080)

The Alkaline Phosphatase (ALPL) Antibody (CAB1080) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the ALPL protein, this antibody enables researchers to detect and analyze ALPL expression in various cell types, making it ideal for studies in bone biology, liver function, and enzyme regulation.

This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Alkaline Phosphatase (ALPL) Antibody
SKU:CAB1080
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:GVKA NEGT VGVS AATE RSRC NTTQ GNEV TSIL RWAK DAGK SVGI VTTT RVNH ATPS AAYA HSAD RDWY SDNE MPPE ALSQ GCKD IAYQ LMHN IRDI DVIM GGGR KYMY PKNK TDVE YESD EKAR GTRL DGLD LVDT WKSF K
Tested Applications:WB IHC-P ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF-P1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HOPS, HPPA, HPPC, HPPI, HPPO, TNAP, TNALP, APTNAP, TNSALP, AP-TNAP, TNS-ALP, Alkaline Phosphatase (ALPL)
Positive Sample:HeLa, Mouse kidney, Rat kidney
Cellular Localization:Cell Membrane, Gpi-Anchor, Lipid-Anchor.
Calculated MW:57kDa
Observed MW:80kDa

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.

Purification MethodAffinity purification
Gene ID249
RRIDAB_2758233
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Alkaline Phosphatase (ALPL) Rabbit pAb (CAB1080) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Alkaline Phosphatase (ALPL) Rabbit pAb (CAB1080) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of paraffin-embedded Mouse colon tissue using Alkaline Phosphatase (ALPL) Rabbit pAb (CAB1080) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Product ImageImmunofluorescence analysis of Human colon cancer tissue using Alkaline Phosphatase (ALPL) Rabbit pAb (CAB1080) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.
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