Developmental Biology

Anti-ALKBH5 Antibody (CAB11684)

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SKU:
CAB11684
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ALKBH5 Antibody
Antibody SKU:CAB11684
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 245-394 of human ALKBH5 (NP_060228.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, Mouse testis, Rat spleen, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 245-394 of human ALKBH5 (NP_060228.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SLPV RRGS VTVL SGYA ADEI THCI RPQD IKER RAVI ILRK TRLD APRL ETKS LSSS VLPP SYAS DRLS GNNR DPAL KPKR SHRK ADPD AAHR PRIL EMDK EENR RSVL LPTH RRRG SFSS ENYW RKSY ESSE DCSE AAGS PARK VKMR RH
Gene ID:54890
Uniprot:Q6P6C2
Cellular Location:Nucleus speckle
Calculated MW:43kDa/44kDa/52kDa
Observed MW:40-52KDa
Synonyms:ALKBH5, ABH5, OFOXD, OFOXD1
Background:
UniProt Protein Function:ALKBH5: a protein contaning a 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily domain. May be involved in DNA alkylation damage repair
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.14.11.-; DNA repair, damage; RNA-binding; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p11.2|17p11.2

Cellular Component: nuclear speck; nucleus

Molecular Function:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors

Biological Process: mRNA export from nucleus; mRNA processing; positive regulation of defense response to virus by host; response to hypoxia; spermatogenesis

UniProt Code:Q6P6C2
NCBI GenInfo Identifier:408359959
NCBI Gene ID:54890
NCBI Accession:Q6P6C2.2
UniProt Secondary Accession:Q6P6C2,Q9NXD6, B4DVJ4, D3DXC6,
UniProt Related Accession:Q6P6C2
Molecular Weight:43,049 Da
NCBI Full Name:RNA demethylase ALKBH5
NCBI Synonym Full Names:alkB homolog 5, RNA demethylase
NCBI Official Symbol:ALKBH5  
NCBI Official Synonym Symbols:ABH5; OFOXD; OFOXD1  
NCBI Protein Information:RNA demethylase ALKBH5
UniProt Protein Name:RNA demethylase ALKBH5
UniProt Synonym Protein Names:Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5
Protein Family:RNA demethylase
UniProt Gene Name:ALKBH5  
UniProt Entry Name:ALKB5_HUMAN
Anti-ALKBH5 Antibody (CAB11684)Western blot analysis of extracts of various cell lines, using ALKBH5 antibody (CAB11684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ALKBH5 Antibody (CAB11684)Western blot analysis of extracts of various cell lines, using ALKBH5 antibody (CAB11684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
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