Cell Biology Antibodies 2

ALDH2 Rabbit Polyclonal Antibody (CAB1226)

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SKU:
CAB1226
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
ALDM, ALDHI, ALDH-E2, ALDH2
Research Use:
Cell Biology
Frequently bought together:

Description

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ALDH2 Antibody (CAB1226)

The ALDH2 Antibody (CAB1226) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is suitable for use in Western blot applications. By binding to the ALDH2 protein, this antibody facilitates the detection and analysis of ALDH2 in a variety of cell types, making it ideal for research in toxicology, pharmacology, and alcohol-related studies.ALDH2 plays a crucial role in protecting cells from oxidative stress and DNA damage by metabolizing toxic aldehydes generated during alcohol metabolism.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ALDH2 Antibody
SKU:CAB1226
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:AGSR TFVQ EDIY DEFV ERSV ARAK SRVV GNPF DSKT EQGP QVDE TQFK KILG YINT GKQE GAKL LCGG GIAA DRGY FIQP TVFG DVQD GMTI AKEE IFGP VMQI LKFK TIEE VVGR ANNS TYGL AAAV FTKD LDKA NYLS QALQ AGTV WVNC YDVF GAQS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ALDM, ALDHI, ALDH-E2, ALDH2
Positive Sample:HepG2, A-549, Mouse liver, Mouse kidney, Rat liver, Rat testis
Cellular Localization:Mitochondrion Matrix.
Calculated MW:56kDa
Observed MW:50-56kDa

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Purification MethodAffinity purification
Gene ID217
RRIDAB_2759126
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using ALDH2 Rabbit pAb (CAB1226) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer using ALDH2 Rabbit pAb (CAB1226) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using ALDH2 Rabbit pAb (CAB1226) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using ALDH2 Rabbit pAb (CAB1226) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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