Cell Death Antibodies 2

Anti-AKTIP Antibody (CAB6239)

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SKU:
CAB6239
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-AKTIP Antibody
Antibody SKU:CAB6239
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human AKTIP (NP_071921.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, MCF7, 293T, Mouse brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human AKTIP (NP_071921.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNPF WSMS TSSV RKRS EGEE KTLT GDVK TSPP RTAP KKQL PSIP KNAL PITK PTSP APAA QSTN GTHA SYGP FYLE YSLL AEFT LVVK QKLP GVYV QPSY RSAL MWFG VIFI RHGL YQDG VFKF TVYI PDNY PDGD CPRL VFDI PVFH PLVD PTSG ELDV KRAF AKWR RNHN HIWQ VLMY ARRV FYKI DTAS PLNP EAAV LYEK DIQL FKSK VVDS VKVC TARL FDQP KIED PYAI SFSP WNPS VHDE AREK MLTQ KKPE EQHN KSVH VAGL SWVK PGSV QPFS KEEK TVAT
Gene ID:64400
Uniprot:Q9H8T0
Cellular Location:Cell membrane, Cytoplasm, Peripheral membrane protein
Calculated MW:33kDa
Observed MW:36kDa
Synonyms:AKTIP, FT1, FTS
Background:The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein.
UniProt Protein Function:AKTIP: an oncogenic membrane-associated protein that is a component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade. Belongs to the ubiquitin-conjugating enzyme family. FTS subfamily. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with HOOK1, HOOK2 and HOOK3. The FHF complex associates with the homotypic vesicular sorting complex (the HOPS complex). Apparently interacts with AKT1. A member of the ubiquitin-conjugating enzyme family, FTS subfamily. Lacks the conserved Cys residue necessary for ubiquitin-conjugating enzyme E2 activity. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Apoptosis; Membrane protein, peripheral; Vesicle

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: plasma membrane

Molecular Function:protein binding; ubiquitin protein ligase binding

Biological Process: early endosome to late endosome transport; endosome organization and biogenesis; endosome to lysosome transport; lysosome organization and biogenesis; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding

NCBI Summary:The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
UniProt Code:Q9H8T0
NCBI GenInfo Identifier:54035954
NCBI Gene ID:64400
NCBI Accession:Q9H8T0.1
UniProt Secondary Accession:Q9H8T0,Q503B1, Q53H38,
UniProt Related Accession:Q9H8T0
Molecular Weight:33kDa
NCBI Full Name:AKT-interacting protein
NCBI Synonym Full Names:AKT interacting protein
NCBI Official Symbol:AKTIP  
NCBI Official Synonym Symbols:FT1; FTS  
NCBI Protein Information:AKT-interacting protein
UniProt Protein Name:AKT-interacting protein
UniProt Synonym Protein Names:Ft1; Fused toes protein homolog
Protein Family:AKT-interacting protein
UniProt Gene Name:AKTIP  
Anti-AKTIP Antibody (CAB6239)Western blot analysis of extracts of various cell lines, using AKTIP antibody (CAB6239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 40s.
Anti-AKTIP Antibody (CAB6239)Immunohistochemistry of paraffin-embedded rat brain using AKTIP antibody (CAB6239) at dilution of 1:100 (40x lens).
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