Metabolism Antibodies 3
Anti-AKR1D1 Antibody (CAB9586)
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- SKU:
- CAB9586
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-AKR1D1 Antibody |
| Antibody SKU: | CAB9586 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human AKR1D1 (NP_005980.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HeLa, HepG2, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human AKR1D1 (NP_005980.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDLS AASH RIPL SDGN SIPI IGLG TYSE PKST PKGA CATS VKVA IDTG YRHI DGAY IYQN EHEV GEAI REKI AEGK VRRE DIFY CGKL WATN HVPE MVRP TLER TLRV LQLD YVDL YIIE VPMA FKPG DEIY PRDE NGKW LYHK SNLC ATWE AMEA CKDA GLVK SLGV SNFN RRQL ELIL NKPG LKHK PVSN QVEC HPYF TQPK LLKF CQQH DIVI TAYS PLGT SRNP IWVN VSSP PLLK DALL NSLG KRYN KTAA QIVL RFNI QRGV VVIP KSFN LERI KENF QIFD FSLT EEEM KDIE ALNK NVRF VELL MWRD HPEY PFHD EY |
| Gene ID: | 6718 |
| Uniprot: | P51857 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 32kDa/37kDa |
| Observed MW: | 30kDa |
| Synonyms: | AKR1D1, 3o5bred, CBAS2, SRD5B1 |
| Background: | The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. |
| UniProt Protein Function: | AKR1D1: Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7- alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4- cholesten-3-one can also act as substrates. Defects in AKR1D1 are the cause of congenital bile acid synthesis defect type 2 (CBAS2); also known as cholestasis with delta(4)-3-oxosteroid 5-beta-reductase deficiency. Patients with this liver disease show absence or low levels of chenodeoxycholic acid and cholic acid in plasma and urine. Belongs to the aldo/keto reductase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - primary bile acid biosynthesis; Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.3.1.3; Lipid Metabolism - C21-steroid hormone Chromosomal Location of Human Ortholog: 7q32-q33 Cellular Component: cytosol Molecular Function:aldo-keto reductase activity; steroid binding Biological Process: androgen metabolic process; bile acid biosynthetic process; C21-steroid hormone metabolic process; cholesterol catabolic process; digestion Disease: Bile Acid Synthesis Defect, Congenital, 2 |
| NCBI Summary: | The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P51857 |
| NCBI GenInfo Identifier: | 1703007 |
| NCBI Gene ID: | 6718 |
| NCBI Accession: | P51857.1 |
| UniProt Secondary Accession: | P51857,A1L4P6, A8K060, B4DPN3, B4DPN8, |
| UniProt Related Accession: | P51857 |
| Molecular Weight: | 32,890 Da |
| NCBI Full Name: | 3-oxo-5-beta-steroid 4-dehydrogenase |
| NCBI Synonym Full Names: | aldo-keto reductase family 1, member D1 |
| NCBI Official Symbol: | AKR1D1 |
| NCBI Official Synonym Symbols: | CBAS2; SRD5B1; 3o5bred |
| NCBI Protein Information: | 3-oxo-5-beta-steroid 4-dehydrogenase |
| UniProt Protein Name: | 3-oxo-5-beta-steroid 4-dehydrogenase |
| UniProt Synonym Protein Names: | Aldo-keto reductase family 1 member D1; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase |
| Protein Family: | 3-oxo-5-beta-steroid 4-dehydrogenase |
| UniProt Gene Name: | AKR1D1 |
| UniProt Entry Name: | AK1D1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AKR1D1 antibody (CAB9586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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