Cell Biology Antibodies 11

Anti-AKR1C4 Antibody (CAB7430)

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SKU:
CAB7430
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-AKR1C4 Antibody
Antibody SKU:CAB7430
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C4 (NP_001809.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, SW620, Mouse liver, Mouse lung, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C4 (NP_001809.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDPK YQRV ELND GHFM PVLG FGTY APPE VPRN RAVE VTKL AIEA GFRH IDSA YLYN NEEQ VGLA IRSK IADG SVKR EDIF YTSK LWCT FFQP QMVQ PALE SSLK KLQL DYVD LYLL HFPM ALKP GETP LPKD ENGK VIFD TVDL SATW EVME KCKD AGLA KSIG VSNF NCRQ LEMI LNKP GLKY KPVC NQVE CHPY LNQS KLLD FCKS KDIV LVAH SALG TQRH KLWV DPNS PVLL EDPV LCAL AKKH KRTP ALIA LRYQ LQRG VVVL AKSY NEQR IREN IQVF EFQL TSED MKVL DGLN RNYR YVVM DFLM DHPD YPFS DEY
Gene ID:1109
Uniprot:P17516
Cellular Location:Cytoplasm
Calculated MW:37kDa
Observed MW:37kDa
Synonyms:AKR1C4, 3-alpha-HSD, C11, CDR, CHDR, DD-4, DD4, HAKRA
Background:This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
UniProt Protein Function:AKR1C4: Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha- androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20- alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. Defects in AKR1C4 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. AKR1C4 mutations may act as modifier of disease severity in SRXY8 patients. A splicing mutation resulting in loss of exon 2 has been found in affected individuals also carrying mutation Val-79 in AKR1C2 (PubMed:21802064). Belongs to the aldo/keto reductase family.
UniProt Protein Details:

Protein type:Lipid Metabolism - androgen and estrogen; Oxidoreductase; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - primary bile acid biosynthesis; EC 1.1.1.357; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.225

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytoplasm; cytosol

Molecular Function:oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; electron carrier activity; aldo-keto reductase activity; chlordecone reductase activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; bile acid transmembrane transporter activity; retinal dehydrogenase activity

Biological Process: steroid metabolic process; bile acid and bile salt transport; bile acid biosynthetic process; phototransduction, visible light; bile acid metabolic process; androgen metabolic process; retinoid metabolic process

Disease: 46,xy Sex Reversal 8

NCBI Summary:This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]
UniProt Code:P17516
NCBI GenInfo Identifier:308153631
NCBI Gene ID:1109
NCBI Accession:P17516.3
UniProt Related Accession:P17516
Molecular Weight:
NCBI Full Name:Aldo-keto reductase family 1 member C4
NCBI Synonym Full Names:aldo-keto reductase family 1 member C4
NCBI Official Symbol:AKR1C4  
NCBI Official Synonym Symbols:C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD  
NCBI Protein Information:aldo-keto reductase family 1 member C4
UniProt Protein Name:Aldo-keto reductase family 1 member C4
UniProt Synonym Protein Names:3-alpha-HSD1; 3-alpha-hydroxysteroid dehydrogenase type I (EC:1.1.1.357); Chlordecone reductase (EC:1.1.1.225); CDR; Dihydrodiol dehydrogenase 4; DD-4; DD4; HAKRA
UniProt Gene Name:AKR1C4  
UniProt Entry Name:AK1C4_HUMAN
Anti-AKR1C4 Antibody (CAB7430)Western blot analysis of extracts of various cell lines, using AKR1C4 antibody (CAB7430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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