KO Validated Antibodies 1

Anti-AKR1B1 Antibody (CAB18031)[KO Validated]

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SKU:
CAB18031
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-AKR1B1 Antibody [KO Validated]
Antibody SKU:CAB18031
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human AKR1B1 (NP_001619.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human AKR1B1 (NP_001619.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MASR LLLN NGAK MPIL GLGT WKSP PGQV TEAV KVAI DVGY RHID CAHV YQNE NEVG VAIQ EKLR EQVV KREE LFIV SKLW CTYH EKGL VKGA CQKT LSDL KLDY LDLY LIHW PTGF KPGK EFFP LDES GNVV PSDT NILD TWAA MEEL VDEG LVKA IGIS NFNH LQVE MILN KPGL KYKP AVNQ IECH PYLT QEKL IQYC QSKG IVVT AYSP LGSP DRPW AKPE DPSL LEDP RIKA IAAK HNKT TAQV LIRF PMQR NLVV IPKS VTPE RIAE NFKV FDFE LSSQ DMTT LLSY NRNW RVCA LLSC TSHK DYPF HEEF
Gene ID:231
Uniprot:P15121
Cellular Location:Cytoplasm
Calculated MW:35kDa
Observed MW:34kDa
Anti-AKR1B1 Antibody [KO Validated] (CAB18031)Western blot analysis of extracts from normal (control) and AKR1B1 knockout (KO) HeLa cells, using AKR1B1 antibody (CAB18031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:AKR1B1, ADR, ALDR1, ALR2, AR
Background:This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database.
UniProt Protein Function:Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
NCBI Summary:This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]
UniProt Code:P15121
NCBI GenInfo Identifier:113596
NCBI Gene ID:231
NCBI Accession:P15121.3
UniProt Secondary Accession:P15121,Q5U031, Q6FGA4, Q6ICP2, Q9BS21, Q9UCI9, B2R8N3
UniProt Related Accession:P15121
Molecular Weight:
NCBI Full Name:Aldose reductase
NCBI Synonym Full Names:aldo-keto reductase family 1 member B
NCBI Official Symbol:AKR1B1  
NCBI Official Synonym Symbols:AR; ADR; ALR2; ALDR1  
NCBI Protein Information:aldose reductase
UniProt Protein Name:Aldose reductase
UniProt Synonym Protein Names:Aldehyde reductase; Aldo-keto reductase family 1 member B1
Protein Family:Aldose reductase
UniProt Gene Name:AKR1B1  
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