Immunology Antibodies 2
Anti-AKAP8 Antibody (CAB3049)
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- SKU:
- CAB3049
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-AKAP8 Antibody |
| Antibody SKU: | CAB3049 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 480-692 of human AKAP8 (NP_005849.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | LO2, 293T, BxPC-3, HeLa, Jurkat, mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 480-692 of human AKAP8 (NP_005849.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HCLA CDML IPAQ PQLL QRHL HSVD HNHN RRLA AEQF KKTS LHVA KSVL NNRH IVKM LEKY LKGE DPFT SETV DPEM EGDD NLGG EDKK ETPE EVAA DVLA EVIT AAVR AVDG EGAP APES SGEP AEDE GPTD TAEA GSDP QAEQ LLEE QVPC GTAH EKGV PKAR SEAA EAGN GAET MAAE AESA QTRV APAP AAAD AEVE QTDA ESKD AVPT E |
| Gene ID: | 10270 |
| Uniprot: | O43823 |
| Cellular Location: | Nucleus matrix |
| Calculated MW: | 76kDa |
| Observed MW: | 110kDa |
| Synonyms: | AKAP8, AKAP 95, AKAP-8, AKAP-95, AKAP95 |
| Background: | This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. |
| UniProt Protein Function: | AKAP8: Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Belongs to the AKAP95 family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; RNA-binding; Mitochondrial; Adaptor/scaffold Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: nucleoplasm; Golgi apparatus; nuclear matrix; membrane; mitochondrion; condensed chromosome; female pronucleus; nucleus Molecular Function:zinc ion binding; double-stranded DNA binding Biological Process: mitosis; mitotic chromosome condensation; signal transduction |
| NCBI Summary: | This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011] |
| UniProt Code: | O43823 |
| NCBI GenInfo Identifier: | 5902752 |
| NCBI Gene ID: | 10270 |
| NCBI Accession: | O43823.1 |
| UniProt Related Accession: | O43823 |
| Molecular Weight: | 692 |
| NCBI Full Name: | A-kinase anchor protein 8 |
| NCBI Synonym Full Names: | A kinase (PRKA) anchor protein 8 |
| NCBI Official Symbol: | AKAP8 |
| NCBI Official Synonym Symbols: | AKAP-8; AKAP95; AKAP 95; AKAP-95 |
| NCBI Protein Information: | A-kinase anchor protein 8; A-kinase anchor protein, 95kDa |
| UniProt Protein Name: | A-kinase anchor protein 8 |
| UniProt Synonym Protein Names: | A-kinase anchor protein 95 kDa; AKAP 95 |
| Protein Family: | A-kinase anchor protein |
| UniProt Gene Name: | AKAP8 |
| UniProt Entry Name: | AKAP8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AKAP8 antibody (CAB3049) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Confocal immunofluorescence analysis of U2OS cells using AKAP8 Polyclonal Antibody (CAB3049) at dilution of 1:200. Blue: DAPI for nuclear staining. |
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