Immunology Antibodies 2
Anti-AKAP8 Antibody (CAB17302)
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- SKU:
- CAB17302
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-AKAP8 Antibody |
| Antibody SKU: | CAB17302 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human AKAP8 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | BT-474, HL-60, SKOV3, HeLa |
| Immunogen: | A synthetic peptide of human AKAP8 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 10270 |
| Uniprot: | O43823 |
| Cellular Location: | Nucleus matrix |
| Calculated MW: | 76kDa |
| Observed MW: | 100kDa |
| Synonyms: | AKAP8, AKAP 95, AKAP-8, AKAP-95, AKAP95 |
| Background: | This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. |
| UniProt Protein Function: | AKAP8: Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Belongs to the AKAP95 family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; RNA-binding; Mitochondrial; Adaptor/scaffold Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: nucleoplasm; Golgi apparatus; nuclear matrix; membrane; mitochondrion; condensed chromosome; female pronucleus; nucleus Molecular Function:zinc ion binding; double-stranded DNA binding Biological Process: mitosis; mitotic chromosome condensation; signal transduction |
| NCBI Summary: | This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011] |
| UniProt Code: | O43823 |
| NCBI GenInfo Identifier: | 5902752 |
| NCBI Gene ID: | 10270 |
| NCBI Accession: | O43823.1 |
| UniProt Related Accession: | O43823 |
| Molecular Weight: | 692 |
| NCBI Full Name: | A-kinase anchor protein 8 |
| NCBI Synonym Full Names: | A kinase (PRKA) anchor protein 8 |
| NCBI Official Symbol: | AKAP8 |
| NCBI Official Synonym Symbols: | AKAP-8; AKAP95; AKAP 95; AKAP-95 |
| NCBI Protein Information: | A-kinase anchor protein 8; A-kinase anchor protein, 95kDa |
| UniProt Protein Name: | A-kinase anchor protein 8 |
| UniProt Synonym Protein Names: | A-kinase anchor protein 95 kDa; AKAP 95 |
| Protein Family: | A-kinase anchor protein |
| UniProt Gene Name: | AKAP8 |
| UniProt Entry Name: | AKAP8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AKAP8 antibody (CAB17302) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat liver using AKAP8 antibody (CAB17302) (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon using AKAP8 antibody (CAB17302) (40x lens). |
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