Cell Biology Antibodies 3

Anti-AKAP6 Antibody (CAB12798)

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SKU:
CAB12798
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AKAP6 Antibody
Antibody SKU:CAB12798
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human AKAP6 (NP_004265.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:U-87MG, SH-SY5Y
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human AKAP6 (NP_004265.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLTM SVTL SPLR SQDL DPMA TDAS PMAI NMTP TVEQ GEGE EAMK DMDS DQQY EKPP PLHT GADW KIVL HLPE IETW LRMT SERV RDLT YSVQ QDSD SKHV DVHL VQLK DICE DISD HVEQ IHAL LETE FSLK LLSY SVNV IVDI HAVQ LLWH QLRV SVLV LRER ILQG LQDA NGNY TRQT DILQ AFSE ETKE GRLD SLTE VDDS GQLT IKCS QNYL SLDC GITA FELS DYSP SEDL LSGL GDMT SSQV KTKP FDSW SYSE MEKE FPEL IR
Gene ID:9472
Uniprot:Q13023
Cellular Location:Nucleus membrane, Sarcoplasmic reticulum
Calculated MW:120kDa/256kDa
Observed MW:105kDa
Synonyms:AKAP6, ADAP100, ADAP6, AKAP100, PRKA6, mAKAP
Background:The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is highly expressed in various brain regions and cardiac and skeletal muscle. It is specifically localized to the sarcoplasmic reticulum and nuclear membrane, and is involved in anchoring PKA to the nuclear membrane or sarcoplasmic reticulum.
UniProt Protein Function:AKAP6: Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the nuclear membrane or sarcoplasmic reticulum. May act as an adapter for assembling multiprotein complexes.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: caveola; cytoplasm; nuclear envelope; perinuclear region of cytoplasm; sarcoplasmic reticulum; T-tubule

Molecular Function:adenylate cyclase binding; protein anchor; protein binding; protein complex scaffold; protein kinase A binding

Biological Process: negative regulation of cAMP biosynthetic process; positive regulation of cell growth; positive regulation of NFAT protein import into nucleus; positive regulation of phosphoprotein phosphatase activity; positive regulation of release of sequestered calcium ion into cytosol; regulation of action potential

NCBI Summary:The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is highly expressed in various brain regions and cardiac and skeletal muscle. It is specifically localized to the sarcoplasmic reticulum and nuclear membrane, and is involved in anchoring PKA to the nuclear membrane or sarcoplasmic reticulum. [provided by RefSeq, Jul 2008]
UniProt Code:Q13023
NCBI GenInfo Identifier:116241243
NCBI Gene ID:9472
NCBI Accession:Q13023.3
UniProt Secondary Accession:Q13023,O15028, A7E242, A7E2D4,
UniProt Related Accession:Q13023
Molecular Weight:257kDa
NCBI Full Name:A-kinase anchor protein 6
NCBI Synonym Full Names:A-kinase anchoring protein 6
NCBI Official Symbol:AKAP6  
NCBI Official Synonym Symbols:ADAP6; PRKA6; mAKAP; ADAP100; AKAP100  
NCBI Protein Information:A-kinase anchor protein 6
UniProt Protein Name:A-kinase anchor protein 6
UniProt Synonym Protein Names:A-kinase anchor protein 100 kDa; AKAP 100; Protein kinase A-anchoring protein 6; PRKA6; mAKAP
Protein Family:A-kinase anchor protein
UniProt Gene Name:AKAP6  
Anti-AKAP6 Antibody (CAB12798)Western blot analysis of extracts of various cell lines, using AKAP6 antibody (CAB12798) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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