Antibody Name:	Anti-AK2 Antibody [KO Validated] (CAB20934)
Antibody SKU:	CAB20934
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunofluorescence
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of Human AK2.

Application:	Western blotting, Immunofluorescence
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver, Rat kidney

Immunogen:	Recombinant protein of Human AK2.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Cellular Location:	Mitochondrion intermembrane space
Calculated MW:	14kDa/21kDa/22kDa/24kDa/25kDa/26kDa
Observed MW:	26KDa

Synonyms:

AK2, ADK2

Background:

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.

	Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of MCF7 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HepG2 cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using [KO Validated] AK2 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
	Western blot analysis of extracts from normal (control) and AK2 knockout (KO) HeLa cells, using AK2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using AK2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.