Cardiovascular Antibodies

Anti-AIMP1 Antibody (CAB5301)

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SKU:
CAB5301
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-AIMP1 Antibody
Antibody SKU:CAB5301
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-180 of human AIMP1 (NP_001135888.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, 22RV1, HepG2, HT-29, MCF7, A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-180 of human AIMP1 (NP_001135888.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MANN DAVL KRLE QKGA EADQ IIEY LKQQ VSLL KEKA ILQA TLRE EKKL RVEN AKLK KEIE ELKQ ELIQ AEIQ NGVK QIPF PSGT PLHA NSMV SENV IQST AVTT VSSG TKEQ IKGG TGDE KKAK EKIE KKGE KKEK KQQS IAGS ADSK PIDV SRLD
Gene ID:9255
Uniprot:Q12904
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Nucleus, Secreted, cytosol, secretory vesicle
Calculated MW:34kDa/37kDa
Observed MW:37kDa
Synonyms:AIMP1, EMAP2, EMAPII, HLD3, SCYE1, p43
Background:The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20.
UniProt Protein Function:Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
NCBI Summary:The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008]
UniProt Code:Q12904
NCBI GenInfo Identifier:85700432
NCBI Gene ID:9255
NCBI Accession:Q12904.2
UniProt Secondary Accession:Q12904,Q6FG28, Q96CQ9, B3KTR2, B4E1S7,
UniProt Related Accession:Q12904
Molecular Weight:37,039 Da
NCBI Full Name:Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
NCBI Synonym Full Names:aminoacyl tRNA synthetase complex interacting multifunctional protein 1
NCBI Official Symbol:AIMP1  
NCBI Official Synonym Symbols:p43; HLD3; EMAP2; SCYE1; EMAPII  
NCBI Protein Information:aminoacyl tRNA synthase complex-interacting multifunctional protein 1
UniProt Protein Name:Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
UniProt Synonym Protein Names:Multisynthase complex auxiliary component p43
Protein Family:Aminoacyl tRNA synthase complex-interacting multifunctional protein
UniProt Gene Name:AIMP1  
UniProt Entry Name:AIMP1_HUMAN
Anti-AIMP1 Antibody (CAB5301)Western blot analysis of extracts of various cell lines, using AIMP1 Antibody (CAB5301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-AIMP1 Antibody (CAB5301)Immunohistochemistry of paraffin-embedded rat spleen using AIMP1 antibody (CAB5301) at dilution of 1:100 (40x lens).
Anti-AIMP1 Antibody (CAB5301)Immunohistochemistry of paraffin-embedded human gastric cancer using AIMP1 antibody (CAB5301) at dilution of 1:100 (40x lens).
Anti-AIMP1 Antibody (CAB5301)Immunohistochemistry of paraffin-embedded mouse heart using AIMP1 antibody (CAB5301) at dilution of 1:100 (40x lens).
Anti-AIMP1 Antibody (CAB5301)Immunofluorescence analysis of HeLa cells using AIMP1 antibody (CAB5301). Blue: DAPI for nuclear staining.
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