Cell Biology Antibodies 2

Anti-AGTRAP Antibody (CAB12178)

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SKU:
CAB12178
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AGTRAP Antibody
Antibody SKU:CAB12178
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 93-152 of human AGTRAP (NP_001035284.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HT-29, U-87MG, BxPC-3, Mouse kidney, Mouse testis, Mouse heart, Rat kidney, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 93-152 of human AGTRAP (NP_001035284.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ILSL LLKP LSCC FVYH MYRE RGGF LGSS QDRS AYQT IDSA EAPA DPFA VPEG RSQD ARGY
Gene ID:57085
Uniprot:Q6RW13
Cellular Location:Cytoplasmic vesicle membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein
Calculated MW:11kDa/16kDa/17kDa
Observed MW:20kDa
Synonyms:AGTRAP, ATRAP
Background:This gene encodes a transmembrane protein localized to the plasma membrane and perinuclear vesicular structures. The gene product interacts with the angiotensin II type I receptor and negatively regulates angiotensin II signaling. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms.
UniProt Protein Function:AGTRAP: Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalisation as well as mechanism of receptor desensitization such as phosphorylation. Induces also a decrease in cell proliferation and angiotensin II-stimulated transcriptional activity. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nucleoplasm

Molecular Function:protein binding

NCBI Summary:This gene encodes a transmembrane protein localized to the plasma membrane and perinuclear vesicular structures. The gene product interacts with the angiotensin II type I receptor and negatively regulates angiotensin II signaling. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q6RW13
NCBI GenInfo Identifier:71152303
NCBI Gene ID:57085
NCBI Accession:Q6RW13.1
UniProt Secondary Accession:Q6RW13,Q5SNV4, Q5SNV5, Q96AC0, Q96PL4, Q9NRW9, A8MVQ5
UniProt Related Accession:Q6RW13
Molecular Weight:11,948 Da
NCBI Full Name:Type-1 angiotensin II receptor-associated protein
NCBI Synonym Full Names:angiotensin II receptor associated protein
NCBI Official Symbol:AGTRAP  
NCBI Official Synonym Symbols:ATRAP  
NCBI Protein Information:type-1 angiotensin II receptor-associated protein
UniProt Protein Name:Type-1 angiotensin II receptor-associated protein
UniProt Synonym Protein Names:AT1 receptor-associated protein
Protein Family:Type-1 angiotensin II receptor-associated protein
UniProt Gene Name:AGTRAP  
UniProt Entry Name:ATRAP_HUMAN
Anti-AGTRAP Antibody (CAB12178)Western blot analysis of extracts of various cell lines, using AGTRAP antibody (CAB12178) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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