Cell Biology Antibodies 10

Anti-AGR2 Antibody (CAB7064)

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SKU:
CAB7064
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-AGR2 Antibody
Antibody SKU:CAB7064
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-175 of human AGR2 (NP_006399.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, BT-474, MCF7, NCI-H460, Mouse stomach, Mouse liver, Mouse small intestine, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-175 of human AGR2 (NP_006399.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RDTT VKPG AKKD TKDS RPKL PQTL SRGW GDQL IWTQ TYEE ALYK SKTS NKPL MIIH HLDE CPHS QALK KVFA ENKE IQKL AEQF VLLN LVYE TTDK HLSP DGQY VPRI MFVD PSLT VRAD ITGR YSNR LYAY EPAD TALL LDNM KKAL KLLK TEL
Gene ID:10551
Uniprot:O95994
Cellular Location:Endoplasmic reticulum, Secreted
Calculated MW:19kDa
Observed MW:20kDa
Synonyms:AGR2, AG-2, AG2, GOB-4, HAG-2, HEL-S-116, HPC8, PDIA17, XAG-2
Background:This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and a C-terminal ER-retention sequence. This protein plays a role in cell migration, cellular transformation and metastasis and is as a p53 inhibitor. As an ER-localized molecular chaperone, it plays a role in the folding, trafficking, and assembly of cysteine-rich transmembrane receptors and the cysteine-rich intestinal gylcoprotein mucin. This gene has been implicated in inflammatory bowel disease and cancer progression.
UniProt Protein Function:AGR2: Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Interacts with LYPD3 and DAG1 (alphaDAG1). Interacts with MUC2; disulfide-linked. Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. Belongs to the AGR family.
UniProt Protein Details:

Protein type:Secreted; Oncoprotein; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p21.3

Cellular Component: endoplasmic reticulum; extracellular space

Molecular Function:epidermal growth factor receptor binding; protein binding; protein homodimerization activity

Biological Process: digestive tract morphogenesis; positive regulation of developmental growth; positive regulation of epidermal growth factor receptor signaling pathway

UniProt Code:O95994
NCBI GenInfo Identifier:67462105
NCBI Gene ID:10551
NCBI Accession:O95994.1
UniProt Related Accession:O95994
Molecular Weight:19,979 Da
NCBI Full Name:Anterior gradient protein 2 homolog
NCBI Synonym Full Names:anterior gradient 2, protein disulphide isomerase family member
NCBI Official Symbol:AGR2  
NCBI Official Synonym Symbols:AG2; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116  
NCBI Protein Information:anterior gradient protein 2 homolog
UniProt Protein Name:Anterior gradient protein 2 homolog
UniProt Synonym Protein Names:HPC8; Secreted cement gland protein XAG-2 homolog
Protein Family:Anterior gradient protein
UniProt Gene Name:AGR2  
UniProt Entry Name:AGR2_HUMAN
Anti-AGR2 Antibody (CAB7064)Western blot analysis of extracts of various cell lines, using AGR2 antibody (CAB7064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-AGR2 Antibody (CAB7064)Immunohistochemistry of paraffin-embedded Human lung cancer using AGR2 Rabbit pAb (CAB7064) at dilution of 1:100 (40x lens).
Anti-AGR2 Antibody (CAB7064)Immunohistochemistry of paraffin-embedded Mouse lung using AGR2 Rabbit pAb (CAB7064) at dilution of 1:100 (40x lens).
Anti-AGR2 Antibody (CAB7064)Immunofluorescence analysis of L929 cells using AGR2 antibody (CAB7064) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-AGR2 Antibody (CAB7064)Immunofluorescence analysis of U-2 OS cells using AGR2 antibody (CAB7064) at dilution of 1:100. Blue: DAPI for nuclear staining.
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system_update_altDatasheetHuman AGR2 Recombinant Protein Anterior Gradient 2 (AGR2) is an 18-21 kDa member of the PDI family of enzymes. AGR2 is widely expressed in secretory cells, such as small